Matrine inhibits mouse sperm function by reducing sperm [Ca2+]i and phospho-ERK1/2

Area of Science:

• Reproductive Biology
• Toxicology
• Pharmacology

Background:

• Matrine is a bioactive alkaloid with diverse pharmacological effects, commonly used in traditional Chinese medicine.
• Its impact on male reproductive health, particularly sperm function, remains largely uncharacterized.
• This study addresses the knowledge gap regarding matrine's in vitro toxicity on mature mouse sperm.

Purpose of the Study:

• To investigate the in vitro toxic effects of matrine on mature mouse sperm.
• To elucidate the mechanisms underlying matrine's potential impact on sperm viability, motility, and fertilization capacity.

Main Methods:

• Mouse cauda epididymal sperm were exposed to varying concentrations of matrine (10-200 µM) in vitro.
• Sperm viability, motility, capacitation, acrosome reaction, and fertilization ability were assessed.
• Intracellular calcium concentration ([Ca(2+)]i), ion channel currents (Catsper, Ksper), and p-ERK1/2 phosphorylation were analyzed.

Main Results:

• Matrine exposure (≥100 µM) significantly reduced sperm motility, velocity, and acrosome reaction rates.
• Fertilization ability of mouse sperm was markedly decreased following matrine treatment.
• Matrine significantly lowered intracellular calcium levels and suppressed p-ERK1/2 phosphorylation, without affecting Catsper and Ksper currents.

Conclusions:

• Matrine inhibits mouse sperm function through a mechanism involving the reduction of intracellular calcium.
• Suppression of extracellular signal-regulated kinases 1/2 (ERK1/2) phosphorylation is implicated in matrine-induced sperm dysfunction.
• These findings highlight potential adverse effects of matrine on male reproductive parameters.