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Employing Digital Droplet PCR to Detect BRAF V600E Mutations in Formalin-fixed Paraffin-embedded Reference Standard Cell Lines
Published on: October 8, 2015
BRAFV600E mutation in papillary thyroid microcarcinoma: a meta-analysis.
Fei Li1, Guangqi Chen2, Chunjun Sheng2
1Department of EndocrinologyShanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Yan Chang Middle Road, Shanghai 200072, ChinaFirst Clinical Medical CollegeNanjing Medical University, Nanjing 210029, ChinaJiangsu Institute of Nuclear MedicineWuxi 214063, ChinaDepartment of CardiologyShanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, ChinaDepartment of Neurology and NeuroscienceWeill Cornell Medical College, New York, New York 10065, USADepartments of Nuclear MedicineUltrasound MedicineShanghai Tenth People's Hospital, Shanghai 200072, ChinaThyroid InstituteTongji University, Shanghai 200072, ChinaDivision of EndocrinologyDiabetes and Metabolism, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA Department of EndocrinologyShanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Yan Chang Middle Road, Shanghai 200072, ChinaFirst Clinical Medical CollegeNanjing Medical University, Nanjing 210029, ChinaJiangsu Institute of Nuclear MedicineWuxi 214063, ChinaDepartment of CardiologyShanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, ChinaDepartment of Neurology and NeuroscienceWeill Cornell Medical College, New York, New York 10065, USADepartments of Nuclear MedicineUltrasound MedicineShanghai Tenth People's Hospital, Shanghai 200072, ChinaThyroid InstituteTongji University, Shanghai 200072, ChinaDivision of EndocrinologyDiabetes and Metabolism, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA Department of EndocrinologyShanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Yan Chang Middle Road, Shanghai 200072, ChinaFirst Clinical Medical CollegeNanjing Medical University, Nanjing 210029, ChinaJiangsu Institute of Nuclear MedicineWuxi 214063, ChinaDepartment of CardiologyShanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, ChinaDepartment o
The BRAFV600E mutation is linked to aggressive features in papillary thyroid microcarcinoma (PTMC). Testing for this mutation aids in risk stratification and management of PTMC patients.
Area of Science:
- Oncology
- Genetics
- Pathology
Background:
- The BRAFV600E mutation is a known poor prognostic marker in papillary thyroid carcinoma.
- Its association with aggressive behavior in papillary thyroid microcarcinoma (PTMC) requires further clarification.
Purpose of the Study:
- To investigate the relationship between BRAFV600E mutation and clinicopathological features in PTMC through a meta-analysis.
- To assess the prognostic value of BRAFV600E in PTMC.
Main Methods:
- Systematic literature search in PubMed, EMBASE, and Cochrane Library.
- Inclusion of 19 studies with 3437 PTMC patients reporting BRAFV600E status.
- Meta-analysis of clinicopathological features and BRAFV600E mutation status.
Main Results:
- BRAFV600E mutation prevalence was 47.48%, with no significant difference by sex or age.
- BRAFV600E mutation was significantly associated with tumor multifocality, extrathyroidal extension, lymph node metastases, and advanced stage.
- Odds ratios indicated increased risk for these aggressive features in mutation-positive PTMC.
Conclusions:
- BRAFV600E-positive PTMC exhibit more aggressive clinicopathological characteristics.
- BRAFV600E mutation testing can assist in risk stratification and management of PTMC.
- This meta-analysis provides definitive evidence for the role of BRAFV600E in PTMC aggressiveness.
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