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Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria
Published on: November 10, 2015
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PlasmoGEM, a database supporting a community resource for large-scale experimental genetics in malaria parasites
Frank Schwach1, Ellen Bushell1, Ana Rita Gomes1
1Wellcome Trust Sanger Institute, Hinxton Cambridge, CB10 1SA, UK.
Nucleic Acids Research
|January 17, 2015
Summary
The Plasmodium Genetic Modification (PlasmoGEM) database offers over 2000 versatile vectors for modifying the Plasmodium berghei parasite genome. These free resources aid researchers in malaria parasite studies and genetic screens.
Area of Science:
- Parasitology
- Genetics
- Bioinformatics
Background:
- Plasmodium parasites cause malaria, necessitating tools for genetic manipulation.
- Genome modification is crucial for understanding parasite biology and developing interventions.
Purpose of the Study:
- To introduce the Plasmodium Genetic Modification (PlasmoGEM) database and its resources.
- To provide researchers with adaptable vectors for Plasmodium spp. genome engineering.
Main Methods:
- Development of over 2000 modular vectors with long homology arms and gene-specific barcodes.
- Implementation of a custom software suite for automated vector design and quality control via sequencing.
- Creation of a web interface for database searching, vector details, and data download.
Main Results:
- PlasmoGEM provides a repository of >2000 plasmids for Plasmodium berghei genome modification.
- Vectors are designed for efficient integration, mutant identification, and diverse applications.
- Automated design and quality control ensure vector reliability.
Conclusions:
- PlasmoGEM is a valuable, free resource for the research community.
- The database facilitates various genome modification applications, including genetic screens.
- Accessible vector design and quality control support advanced Plasmodium research.
