Related Experiment Video

Updated: Apr 18, 2026

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines
08:50

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

1.1K

SRrp35 suppresses cell proliferation and malignancy in hepatocellular carcinoma

Yandong Li1,2, Yun Lin1, Qun Li1

  • 1Department of Oncology, Shanghai, China.

Hepatology Research : the Official Journal of the Japan Society of Hepatology
|January 17, 2015
PubMed
Abstract

Insights

Serine/arginine-rich protein 35 (SRrp35) suppresses hepatocellular carcinoma (HCC) cell growth and malignancy. Downregulation of SRrp35 is common in HCC, suggesting it is a potential therapeutic target for liver cancer.

Area of Science:

  • Molecular biology
  • Cancer research
  • RNA splicing

Background:

  • Serine/arginine-rich (SR) proteins regulate alternative pre-mRNA splicing, RNA metabolism, and cancer development.
  • SRrp35 antagonizes classical SR proteins in splicing regulation, but its role in cancer is unclear.

Purpose of the Study:

  • Investigate the function of SRrp35 in hepatocellular carcinoma (HCC).

Main Methods:

  • Quantitative real-time PCR to assess SRrp35 expression in HCC samples and cell lines.
  • In vitro assays (cell viability, colony formation, soft agar growth) and in vivo tumorigenicity assays in nude mice.

Main Results:

  • SRrp35 was downregulated in 62.5% of HCC specimens and most HCC cell lines.
  • Enhanced SRrp35 expression suppressed HCC cell proliferation and colony formation.
  • SRrp35 knockdown promoted proliferation and colony formation; in vivo studies confirmed its tumor-suppressive role.

Conclusions:

  • SRrp35 suppresses cell proliferation and malignancy in HCC.
  • SRrp35 represents a potential therapeutic target for hepatocellular carcinoma.
Keywords:
SRrp35cell proliferationhepatocellular carcinomamalignancy

More Related Videos

An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis06:38

An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis

Published on: September 12, 2019

9.7K
Stem Cell-Derived Viral Ag-Specific T Lymphocytes Suppress HBV Replication in Mice07:25

Stem Cell-Derived Viral Ag-Specific T Lymphocytes Suppress HBV Replication in Mice

Published on: September 25, 2019

7.5K

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.5K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
5.0K
Replicative Cell Senescence02:15

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
4.7K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
39.2K
The Ras Gene02:38

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
7.6K
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
6.7K