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Updated: Apr 18, 2026

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture
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Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture

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Cellular disulfide bond formation in bioactive peptides and proteins

Nitin A Patil1, Julien Tailhades2, Richard Anthony Hughes3

  • 1Florey Institute of Neuroscience and Mental Health, the University of Melbourne, Victoria 3010, Australia. fs@unimelb.edu.au.

International Journal of Molecular Sciences
|January 17, 2015
PubMed
Summary

Cellular machinery forms disulfide bonds crucial for bioactive peptide structure and function. This review details the enzymes and pathways involved in disulfide bond generation and maintenance in both prokaryotes and eukaryotes.

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