Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nucleotide Excision Repair01:38

Nucleotide Excision Repair

6.0K
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
6.0K
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

42.9K
Overview
42.9K
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

14.1K
14.1K
Translesion DNA Polymerases02:10

Translesion DNA Polymerases

11.9K
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
11.9K
Homologous Recombination02:31

Homologous Recombination

66.0K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
66.0K
Homologous Recombination02:31

Homologous Recombination

7.7K
7.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>Porphyromonas gingivalis</i> secreted factors drive epithelial-mesenchymal transition (EMT) through gingipains and an H<sub><b>2</b></sub>S-mediated bacterial defense system.

Gut microbes·2026
Same author

ZCWPW1 organizes telomeric architecture to drive meiotic chromosome movements.

bioRxiv : the preprint server for biology·2026
Same author

Author Correction: Unravelling cysteine-deficiency-associated rapid weight loss.

Nature·2025
Same author

Collateral sensitivity and genetic vulnerability of antibiotic resistance.

Trends in microbiology·2025
Same author

Molecular basis for noncanonical transcription initiation from Np<sub>4</sub>A alarmones.

Nature chemical biology·2025
Same author

Elementary 3D organization of active and silenced E. coli genome.

Nature·2025

Related Experiment Video

Updated: Apr 18, 2026

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

14.1K

Rethinking transcription coupled DNA repair.

Venu Kamarthapu1, Evgeny Nudler1

  • 1Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY 10016, USA; Howard Hughes Medical Institute, New York University School of Medicine, New York, NY 10016, USA.

Current Opinion in Microbiology
|January 18, 2015
PubMed
Summary

A new bacterial transcription-coupled repair (TCR) pathway utilizes UvrD and NusA to initiate DNA damage repair. This finding offers insights into genome integrity maintenance and contrasts with conventional TCR models.

More Related Videos

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
06:59

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Published on: March 31, 2022

2.9K
Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
08:31

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Published on: June 8, 2018

9.8K

Related Experiment Videos

Last Updated: Apr 18, 2026

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

14.1K
Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
06:59

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Published on: March 31, 2022

2.9K
Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
08:31

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Published on: June 8, 2018

9.8K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Nucleotide excision repair (NER) is a crucial DNA repair mechanism.
  • Transcription-coupled repair (TCR) is a subpathway of NER that prioritizes repairing the transcribed DNA strand.
  • RNA polymerase (RNAP) stalling at DNA lesions is a key signal for TCR initiation.

Purpose of the Study:

  • To review a newly identified bacterial TCR pathway.
  • To highlight the roles of UvrD and NusA in initiating bacterial TCR.
  • To compare new and conventional TCR models and discuss their operation.

Main Methods:

  • Literature review focusing on bacterial DNA repair mechanisms.
  • Comparative analysis of different TCR pathway models.
  • Discussion of the role of pervasive transcription in genome maintenance.

Main Results:

  • Identification of a novel bacterial TCR pathway involving UvrD and NusA.
  • Elucidation of the initiation mechanism of this new TCR pathway.
  • Comparison of the functional trade-offs between different TCR models.

Conclusions:

  • The UvrD-NusA pathway represents a significant addition to our understanding of bacterial DNA repair.
  • Understanding TCR pathways is essential for comprehending genome integrity maintenance.
  • Pervasive transcription plays a vital role in protecting the bacterial genome from damage.