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Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
Published on: April 12, 2019
Evaluation of the formation of a junctional DNA nanostructure through annealing curve analysis
Seung Won Shin1, Kyung Soo Park1, Soong Ho Um2
1School of Chemical Engineering, Sungkyunkwan University, Suwon, 440-746, South Korea.
Abstract:
During the self-assembly of different numbers of oligonucleotides comprising junctional DNA nanostructures, a change in environmental variables (e.g., temperature or salt concentration) has a substantial influence on the final products. Further, distinctive annealing temperatures of oligonucleotides are observed depending on the state of hybridization. Here, we present an evaluation of the annealing characteristics of oligonucleotides for the formation of a simple junctional DNA nanostructure using an annealing curve analysis. This method may be useful for analyzing the formation of complex junctional DNA nanostructures.
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