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Plasma cholinesterase activity of rats, western grey kangaroos, alpacas, sheep, cattle, and horses
Chris Mayberry1, Peter Mawson2, Shane K Maloney3
1School of Animal Biology, The University of Western Australia, Stirling Highway, Crawley 6009, Western Australia, Australia.
Introduction:
Plasma cholinesterase activity levels of various species may be of interest to toxicologists or pathologists working with chemicals that interfere with the activity of plasma cholinesterase.
Methods:
We used a pH titration method to measure the plasma cholinesterase activity of six mammalian species.
Results:
Plasma cholinesterase activity varied up to 50-fold between species: sheep (88 ± 45 nM acetylcholine degraded per ml of test plasma per minute), cattle (94 ± 35), western grey kangaroos (126 ± 92), alpaca (364 ± 70), rats (390 ± 118) and horses (4539 ± 721).
Discussion:
We present a simple, effective technique for the assay of plasma cholinesterase activity levels from a range of species. Although labour-intensive, it requires only basic laboratory equipment.
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