Related Experiment Video

Updated: Apr 18, 2026

Investigating Target Gene Function in a CD40 Agonistic Antibody-induced Colitis Model using CRISPR/Cas9-based Technologies
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Investigating Target Gene Function in a CD40 Agonistic Antibody-induced Colitis Model using CRISPR/Cas9-based Technologies

Published on: June 2, 2021

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MS risk allele rs1883832T is associated with decreased mRNA expression of CD40

Marta Wagner1, Maciej Sobczyński, Małgorzata Bilińska

  • 1Laboratory of Immunogenetics and Tissue Immunology, Department of Clinical Immunology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, ul. Weigla 12, 53-114, Wrocław, Poland, marta_wagner@o2.pl.

Journal of Molecular Neuroscience : MN
|January 21, 2015
PubMed

Insights

Investigating CD40 and CD40L gene expression in multiple sclerosis (MS) revealed a link between specific CD40 gene variants and reduced CD40 mRNA levels. This suggests impaired CD40-CD40L interactions may contribute to MS development.

Area of Science:

  • Immunology
  • Genetics
  • Neuroscience

Background:

  • CD40-CD40L interactions are crucial for immune responses involving T and B cells.
  • These interactions are implicated in autoimmune diseases like multiple sclerosis (MS), characterized by activated immune cells.

Purpose of the Study:

  • To analyze CD40 and CD40L mRNA expression in MS patients and controls.
  • To investigate the impact of specific CD40 and CD40L single nucleotide polymorphisms (SNPs) on gene expression.

Main Methods:

  • Analysis of CD40 and CD40L mRNA expression in whole blood samples.
  • Examination of the association between selected CD40 and CD40L SNPs and their respective mRNA levels.

Main Results:

  • The CD40 rs1883832C>T SNP significantly affects CD40 gene expression.
  • Individuals with CT and TT genotypes at rs1883832, associated with MS predisposition, exhibited lower CD40 mRNA levels compared to CC genotype.

Conclusions:

  • Impaired CD40-CD40L interaction is potentially involved in the pathogenesis of multiple sclerosis.
  • The rs1883832C>T polymorphism in CD40 may influence MS susceptibility through altered gene expression.

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