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Specific identification of Bordetella pertussis by the polymerase chain reaction
1Service for Applied Genetics, Université Libre de Bruxelles, Nivelles, Belgium.
Research in Microbiology
|September 1, 1989
Abstract:
Oligonucleotide primers were used to amplify specific DNA regions of the Bordetella pertussis genome by the polymerase chain reaction. One pair of primers, PTp1/PTp2, identified a 191-bp DNA fragment located in the regulatory region of the pertussis toxin operon; a second pair of primers led to amplification of a 121-bp DNA piece located in an insertion-like element specific to B. pertussis. Both sets of primers were able to discriminate between the pathogen and related Bordetella species; they detected down to 6 bacteria and appeared suitable for routine detection of B. pertussis in clinical specimens.