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Updated: Apr 18, 2026

Microplot Design and Plant and Soil Sample Preparation for 15Nitrogen Analysis
Published on: May 10, 2020
Does inorganic nitrogen fertilization improve soil aggregation? Insights from two long-term tillage experiments
Long-term nitrogen fertilization impacts soil aggregation differently based on tillage and crop type. While it improves surface aggregation in no-till continuous corn, subsoil aggregation may decline, with minimal deterioration at approximately 80 kg N/ha.
Area of Science:
- Agricultural Science
- Soil Science
- Agronomy
Background:
- Understanding the effects of inorganic fertilization on soil aggregation is crucial for sustainable agriculture.
- Long-term studies are needed to elucidate the complex interactions between nitrogen (N) fertilization, tillage, and soil structure.
Purpose of the Study:
- To investigate the cumulative impacts of nitrogen fertilization on soil aggregation, soil organic carbon (SOC), and pH.
- To examine these effects across different tillage systems (chisel plow, ridge till, moldboard plow, reduced till, no-till) and cropping systems (continuous corn, corn-soybean) under irrigated and rainfed conditions.
Main Methods:
- Field experiments were conducted over 27-28 years in Nebraska, applying various rates of N fertilizer (0-300 kg N/ha) to continuous corn and corn-soybean rotations.
- Soil samples were analyzed for mean weight diameter of water-stable aggregates (MWDA), SOC, and pH at different depths.
Main Results:
- Nitrogen fertilization altered soil aggregation in continuous corn across tillage systems.
- MWDA increased in the near-surface (0-7.5 cm) in no-till but decreased significantly in deeper layers (7.5-60 cm) with N application.
- Soil pH decreased with N fertilization, while SOC showed little change. No-till and reduced till systems exhibited superior near-surface aggregation compared to moldboard plow.
Conclusions:
- Long-term nitrogen fertilization can enhance near-surface soil aggregation in no-till continuous corn systems but may negatively impact subsoil aggregation.
- Applying N fertilizer at rates around 80 kg N/ha appears to minimize soil aggregation deterioration.
- Tillage practices significantly influence the response of soil aggregation to N fertilization.
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