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The Caco-2 Cell Bioassay for Measurement of Food Iron Bioavailability
Published on: April 28, 2022
[Development and application of Caco-2 cell lines transfering model for assessing the bioavailability of mycotoxins]
Xiaohui Lin1, Xiao Hu, Fengqin Li
1Key Laboratory of Food Safety Risk Assessment of Health, China National Center for Food Safety Risk Assessment, Beijing 100021, China. 821414@163.com
Objective:
The Caco-2 cell model for assessing the bioavailabilities of mycotoxins was developed and validated.
Methods:
The Caco-2 monolayer cell transferring model was developed and validated by optimizing the initial concentrations of toxins in the apical side, the temperature and pH values of the experiment system, and the presence of mycotoxin adsorbent.
Results:
The apparent permeability coefficient (Papp) of AFB1 reached the highest when pH value was in the range between 7.4 and 7.5, while the Papps for DON, AFB2, AFG1 and AFG2 were at the highest in the pH value between 7.0 and 7.1, respectively. The permeabilities of these five mycotoxins were increasing along with the temperature ascending. The transmicivities of AFB1, AFG1 and AFG2 reached the highest at the incubation time of 60 minutes but 120 minutes for DON and AFB2.
Conclusion:
The developed Caco-2 transferring model of the five mycotoxins was susceptible, stable, effective and reproducible.
