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Effect of resveratrol on restoring spermatogenesis in experimental cryptorchid mice and analysis of related
Enzhong Li1,2, Yuping Guo3, Gailing Wang1
1Huanghuai University, 6 Kaiyuan Road, Zhumadian, 463000, China.
Abstract:
The present study aimed to evaluate the effect of trans-Resveratrol on spermatogenesis. Male Kunming suckling mice (10 days old) were surgically rendered cryptorchid and subcutaneously injected with trans-Resveratrol at doses of 5, 10, 20, and 40 µg/g/day as groups I, II, III, and IV, respectively, for 35 days. Animals in the control group received 10 µL/mouse/day of olive oil. Serum estradiol, testosterone, FSH, and LH levels were measured on day 45. Tissue analysis and sperm morphological abnormalities analysis were done. Results showed that in the control group and group I only spermatogonia and primary spermatocytes were present, whereas spermatogenesis was totally restored in groups II, III, and IV. Sperm counts in groups III and IV were remarkably higher than the control group (P<0.05). The morphological abnormalities in resveratrol-treated groups were higher than the mature mice. Serum estradiol levels in the resveratrol-treated groups were not significantly different from the control group, but were lower than the mature mice (P<0.05). There was no significant difference in serum testosterone levels between the resveratrol-treated groups and mature mice, but the levels in the resveratrol-treated groups was significantly lower than the control group (P<0.05). No significant influence of trans-Resveratrol was observed on serum FSH levels in all cryptorchid mice. Serum LH levels in groups I, II, and III were higher than the control group. These results indicate that trans-Resveratrol restores spermatogenesis in cryptorchid mice. In addition, proteomic analysis between the 20 μg/g/day resveratrol-treated group and the control group was carried out, and five kinds of proteins (BAF250, ZFP261, CHD1L, RBBP9, and SOHLH2) were identified. The expression of SOHLH2 increased, while that of BAF250, ZFP261, CHD1L, and RBBP9 decreased in the 20 µg/g/day resveratrol-treated group, indicating that SOHLH2 may contribute to testicular germ cell differentiation.
Insights
Trans-resveratrol treatment restored spermatogenesis in cryptorchid mice. This compound significantly increased sperm counts and influenced protein expression, suggesting a role in testicular germ cell differentiation.
Area of Science:
- Reproductive Biology
- Endocrinology
- Molecular Biology
Background:
- Cryptorchidism, the failure of testes to descend, can lead to impaired spermatogenesis and infertility.
- Trans-resveratrol, a natural polyphenol, has demonstrated various biological activities, including antioxidant and anti-inflammatory effects.
- Evaluating the therapeutic potential of trans-resveratrol in reproductive health is of significant interest.
Purpose of the Study:
- To investigate the efficacy of trans-resveratrol in restoring spermatogenesis in a cryptorchid mouse model.
- To analyze the effects of trans-resveratrol on sperm parameters, hormone levels, and testicular tissue.
- To explore the molecular mechanisms underlying trans-resveratrol's action through proteomic analysis.
Main Methods:
- Male Kunming mice underwent cryptorchid surgery and were treated with varying doses of trans-resveratrol (5-40 µg/g/day) or olive oil (control) for 35 days.
- Serum hormone levels (estradiol, testosterone, FSH, LH) were measured.
- Sperm counts and morphological abnormalities were assessed.
- Proteomic analysis was performed on testicular tissue from the highest dose group.
Main Results:
- Trans-resveratrol treatment dose-dependently restored spermatogenesis in cryptorchid mice, with significant increases in sperm counts at higher doses.
- While morphological abnormalities were observed, trans-resveratrol did not significantly alter estradiol or FSH levels.
- Testosterone levels were not significantly different from mature mice but were lower than controls, and LH levels increased in lower-dose groups. Proteomic analysis revealed altered expression of key proteins including increased SOHLH2.
Conclusions:
- Trans-resveratrol effectively restores spermatogenesis in cryptorchid mice, indicating its potential as a therapeutic agent.
- The compound influences key proteins involved in testicular germ cell differentiation, notably SOHLH2.
- Further research is warranted to elucidate the precise molecular pathways and optimize therapeutic strategies.
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