Related Experiment Video

Updated: Apr 18, 2026

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

15.1K

Oncoprotein YAP regulates the spindle checkpoint activation in a mitotic phosphorylation-dependent manner through

Shuping Yang1, Lin Zhang2, Xingcheng Chen2

  • 1From the Department of Oncology, Shandong Provincial Hospital affiliated with Shandong University, Jinan, Shandong 250021, China and the Eppley Institute for Research in Cancer and Allied Diseases, Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, Nebraska 68198.

The Journal of Biological Chemistry
|January 22, 2015
PubMed

Insights

Yes-associated protein (YAP) regulates the spindle checkpoint, a critical cell division process. YAP

Area of Science:

  • Cell Biology
  • Molecular Oncology
  • Cancer Research

Background:

  • The transcriptional co-activator YAP (Yes-associated protein) is recognized as an oncogene.
  • The precise mechanisms through which YAP contributes to oncogenesis, particularly its role in cell division regulation, remain incompletely understood.

Purpose of the Study:

  • To investigate the functional significance of YAP and its mitotic phosphorylation in the context of the spindle checkpoint.
  • To elucidate the molecular mechanisms by which YAP influences spindle checkpoint activation and mitotic progression.
  • To explore the role of YAP in promoting cancer progression, specifically epithelial cell invasion.

Main Methods:

  • Investigated YAP's dynamic mitotic phosphorylation, identifying CDC14 dependence.
  • Assessed YAP's requirement for spindle checkpoint activation by spindle poisons.
  • Examined the impact of YAP expression levels and mitotic phosphorylation on spindle checkpoint activity and mitotic defects.
  • Analyzed YAP's transcriptional control over spindle checkpoint genes and its interaction with BubR1 (BUB1-related protein kinase).
  • Studied YAP's role in epithelial cell invasion through phosphorylation and BubR1-dependent pathways.

Main Results:

  • YAP's dynamic mitotic phosphorylation is dependent on CDC14.
  • YAP is essential for spindle checkpoint activation induced by spindle poisons, with mitotic phosphorylation being crucial.
  • Enhanced expression of active YAP leads to hyperactivation of the spindle checkpoint and mitotic defects in a phosphorylation-dependent manner.
  • Mitotic phosphorylation of YAP regulates the transcription of spindle checkpoint genes.
  • YAP interacts with BubR1, and BubR1 knockdown mitigates YAP-induced spindle checkpoint hyperactivation.
  • YAP promotes epithelial cell invasion via both mitotic phosphorylation and BubR1-dependent pathways.

Conclusions:

  • This study reveals a novel functional link between YAP and the spindle checkpoint.
  • YAP's oncogenic function may be mediated through the dysregulation of the spindle checkpoint.
  • Mitotic phosphorylation of YAP and its interaction with BubR1 are key mechanisms underlying its role in cell division and invasion.
Keywords:
BubR1Hippo PathwayMitosisOncogenePhosphorylationSpindle CheckpointYes-associated Protein (YAP)

More Related Videos

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast08:13

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

749
Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

7.5K

Related Concept Videos

The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
4.0K
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

2.0K
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
3.6K
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

1.9K
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
4.8K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
39.2K