Related Experiment Video
Updated: Apr 18, 2026

A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals
Published on: March 13, 2011
Simple sequence repeat markers for the endangered species Clianthus puniceus and C. maximus (Fabaceae)
Gary J Houliston1, Ana Ramón-Laca1, Reema Jain1
1Landcare Research, Gerald Street, Lincoln 7640, New Zealand.
Premise Of The Study:
Microsatellite markers were developed for Clianthus puniceus using a shotgun sequencing library and tested for cross amplification in the closely related C. maximus to inform population management of these two endangered species. •
Methods And Results:
We constructed a shotgun sequencing library using a Roche 454 sequencer and searched the resulting data set for putative microsatellite regions. We optimized 12 of these regions to produce polymorphic markers for Clianthus. We tested these markers on four populations of C. maximus and on four C. puniceus individuals of known provenance. Alleles per locus ranged from two to nine, while observed and expected heterozygosities per locus ranged from 0.000 to 1.000 and 0.178 to 0.600, respectively. •
Conclusions:
These markers will be valuable for ongoing monitoring of the genetic variation in naturally occurring populations of Clianthus and for the selection of individuals for revegetation projects in the species' former range.
More Related Videos
08:54Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
Published on: November 5, 2020
10:01Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization FISH with PNA Probes in Caenorhabditis elegans
Published on: August 4, 2016
Related Concept Videos
Multi-species Conserved Sequences
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
Non-LTR Retrotransposons
Cis-regulatory Sequences