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Related Experiment Videos

Voltage dependent calcium channel expression in isolated osteoclasts.

A Teti, M Grano, S Colucci

    Bollettino Della Societa Italiana Di Biologia Sperimentale
    |December 1, 1989
    PubMed
    Summary

    Osteoclasts possess voltage-dependent calcium channels sensitive to depolarization. These channels, likely L-type, were effectively blocked by nicardipine, indicating their role in osteoclast function.

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    Area of Science:

    • Cellular Biology
    • Physiology
    • Pharmacology

    Background:

    • Osteoclasts are crucial for bone resorption.
    • The role of calcium signaling in osteoclast activity is significant.
    • Voltage-dependent calcium channels (VDCCs) are implicated in various cellular functions.

    Purpose of the Study:

    • To investigate the presence and characteristics of VDCCs on osteoclast plasma membranes.
    • To determine the functional role of these channels in osteoclasts.
    • To identify the specific subtypes of VDCCs expressed by osteoclasts.

    Main Methods:

    • Osteoclast isolation and culture.
    • KCl-induced depolarization to stimulate calcium influx.
    • Measurement of intracellular calcium concentration ([Ca2+]i) changes.

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  • Pharmacological inhibition of VDCCs using specific antagonists (nicardipine, verapamil, diltiazem).

    • Main Results:

    • Osteoclasts exhibited sensitivity to KCl-induced depolarization, leading to a transient, dose-dependent increase in [Ca2+]i.
    • The half-maximal effect for KCl-induced calcium increase was observed at 30 mM.
    • Nicardipine, a dihydropyridine, was a potent inhibitor, completely blocking channels at 10(-6) M.
    • Verapamil and diltiazem showed less efficacy in blocking these channels.

    Conclusions:

    • Osteoclasts express functional voltage-dependent calcium channels on their plasma membrane.
    • The results strongly suggest the presence of L-type VDCCs in osteoclasts.
    • These findings contribute to understanding calcium's role in osteoclast physiology and potential therapeutic targets.