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Updated: Apr 18, 2026

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

Published on: March 2, 2012

Capacitive deionization on-chip as a method for microfluidic sample preparation.

Susan H Roelofs¹, Bumjoo Kim, Jan C T Eijkel

¹BIOS - the Lab-on-a-Chip group, Mesa+ Institute for Nanotechnology, MIRA Institute, University of Twente, P.O. box 217, 7500 AE Enschede, The Netherlands. s.h.roelofs@utwente.nl.

|January 22, 2015

Summary

This summary is machine-generated.

Capacitive deionization (CDI) on a chip effectively removes salts and buffers from samples, crucial for accurate mass spectrometry in medical research. This method preserves larger molecules and introduces real-time monitoring via impedance spectroscopy.

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Published on: November 23, 2015

Area of Science:

Analytical Chemistry
Electrochemistry
Biotechnology

Background:

Sample preparation is critical for reproducible mass spectrometry, especially in medical research and clinical diagnostics.
Salts and buffers in biological samples increase noise and reduce signal-to-noise ratio, hindering accurate protein analysis.
Capacitive deionization (CDI) is an electrostatic technique for ion removal from solutions using porous electrodes.

Purpose of the Study:

To demonstrate the feasibility of capacitive deionization (CDI) on a chip for sample preparation.
To assess the efficiency of CDI in removing ions while preserving larger biomolecules.
To introduce in situ, real-time monitoring of desalination using impedance spectroscopy.

Main Methods:

Fabrication and implementation of a microfluidic chip for capacitive deionization.
Application of a 0.5 V potential to drive ion migration and storage in electrode double layers.
Utilizing impedance spectroscopy to monitor ion concentration changes during the desalination process.

Main Results:

Achieved a 23% reduction in sodium (Na+) and chloride (Cl-) ion concentrations.
Demonstrated no significant change in the concentration of larger molecules, such as FITC-dextran.
Successfully implemented impedance spectroscopy for in situ, real-time monitoring of salt concentration.

Conclusions:

Capacitive deionization on a chip is a viable technique for sample preparation in mass spectrometry.
The CDI method effectively removes small ions without affecting larger biomolecules, enhancing analytical accuracy.
Impedance spectroscopy provides a novel, real-time method for monitoring desalination efficiency.