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Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

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Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
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Related Experiment Video

Updated: Apr 18, 2026

Intraductal Delivery and X-ray Visualization of Ethanol-Based Ablative Solution for Prevention and Local Treatment of Breast Cancer in Mouse Models
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Injectable Colloidal Gold for Use in Intrafractional 2D Image-Guided Radiation Therapy.

Rasmus I Jølck1, Jonas S Rydhög, Anders N Christensen

  • 1DTU Nanotech, Department of Micro-and Nanotechnology, Center for Nanomedicine and Theranostics Technical University of Denmark, Building 345E, Ørsteds Plads, 2800, Lyngby, Denmark.

Advanced Healthcare Materials
|January 22, 2015
PubMed
Summary

A new injectable liquid fiducial marker enhances radiation therapy precision for cancer patients. This gold nanoparticle-based marker improves image-guided radiotherapy (IGRT) accuracy and tumor monitoring.

Keywords:
computed tomographyfiducial markersgold nanoparticlesimage-guided radiation therapysucrose acetate isobutyrate

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Area of Science:

  • Biomedical Engineering
  • Oncology
  • Nanotechnology

Background:

  • Radiotherapy is a common cancer treatment, often requiring precise targeting.
  • Image-guided radiotherapy (IGRT) improves dose delivery accuracy.
  • Fiducial markers are used to enhance IGRT precision and monitor tumor position.

Purpose of the Study:

  • To present a novel, injectable liquid fiducial tissue marker for enhanced radiotherapy.
  • To evaluate the biocompatibility, stability, and imaging properties of the liquid fiducial.
  • To assess the clinical potential of the marker in image-guided radiation therapy.

Main Methods:

  • Surface-engineering gold nanoparticles with a carbohydrate-based gelation matrix to create a radio-opaque liquid fiducial.
  • Injecting the liquid fiducial into tumor tissue using thin needles.
  • Testing biocompatibility and stability in mice.
  • Evaluating clinical potential in a canine cancer patient with a spontaneous solid tumor.

Main Results:

  • The liquid fiducial demonstrated high radio-opacity for 2D and 3D X-ray imaging.
  • The marker was highly biocompatible and stable after implantation in mice.
  • Successful injection and image-guided radiation treatment alignment in a canine cancer patient.
  • The marker facilitated precise alignment and imaging during radiation therapy.

Conclusions:

  • The novel liquid fiducial marker exhibits promising properties for improving radiotherapy precision.
  • The marker's high radio-opacity and biocompatibility support its use in image-guided radiotherapy.
  • Further investigation in human cancer patients is warranted to explore its clinical utility.