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Related Experiment Video

Updated: Apr 18, 2026

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

A lateral electrophoretic flow diagnostic assay.

Robert Lin¹, Arunan Skandarajah, Rachel E Gerver

¹Department of Bioengineering, UC Berkeley, Berkeley, CA 94720 USA. aeh@berkeley.edu.

|January 23, 2015

Summary

This summary is machine-generated.

We developed a novel lateral e-flow assay using electrophoresis for rapid, multiplexed disease screening. This assay successfully detected Hepatitis C virus (HCV) antibodies in human serum within 60 minutes.

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Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology

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Published on: March 22, 2012

Area of Science:

Biotechnology
Assay Development
Microfluidics

Background:

Immunochromatographic assays are vital for disease screening.
Existing lateral flow assays rely on passive wicking flow.
There is a need for enhanced multiplexed detection formats.

Purpose of the Study:

To introduce a novel lateral flow assay format using directed electrophoretic transport.
To enable multiplexed detection of biomarkers.
To develop a rapid diagnostic tool for near-patient settings.

Main Methods:

Fabrication of lateral e-flow devices using mask-based UV photopatterning.
Selective immobilization of unmodified capture antigen within microchannels.
Utilizing low-power electrophoresis for biospecimen transport.
Heterogeneous sandwich assay with fluorescence barcode readout via CellScope imaging.

Main Results:

Demonstrated a serum assay for antibodies to the hepatitis C virus (HCV).
Successfully identified HCV-positive human sera in a pilot study within 60 minutes.
Characterized the performance of the lateral e-flow assay.

Conclusions:

The lateral e-flow assay offers a flexible platform for multiplexed immunoassays.
This technology is suitable for confirmatory diagnosis in near-patient settings.
Electrophoretic transport enhances lateral flow assay capabilities for disease screening.