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Published on: May 5, 2018
NLP-12 engages different UNC-13 proteins to potentiate tonic and evoked release
Zhitao Hu1, Amy B Vashlishan-Murray2, Joshua M Kaplan3
1Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, and.
Abstract:
A neuropeptide (NLP-12) and its receptor (CKR-2) potentiate tonic and evoked ACh release at Caenorhabditis elegans neuromuscular junctions. Increased evoked release is mediated by a presynaptic pathway (egl-30 Gαq and egl-8 PLCβ) that produces DAG, and by DAG binding to short and long UNC-13 proteins. Potentiation of tonic ACh release persists in mutants deficient for egl-30 Gαq and egl-8 PLCβ and requires DAG binding to UNC-13L (but not UNC-13S). Thus, NLP-12 adjusts tonic and evoked release by distinct mechanisms.
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