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Published on: April 21, 2012
Improving Leishmania species identification in different types of samples from cutaneous lesions
Mónica L Cruz-Barrera1, Clemencia Ovalle-Bracho2, Viviana Ortegon-Vergara2
1Laboratorio de Parasitología, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá, Colombia.
Abstract:
The discrimination of Leishmania species from patient samples has epidemiological and clinical relevance. In this study, different gene target PCR-restriction fragment length polymorphism (RFLP) protocols were evaluated for their robustness as Leishmania species discriminators in 61 patients with cutaneous leishmaniasis. We modified the hsp70-PCR-RFLP protocol and found it to be the most reliable protocol for species identification.
