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Related Experiment Video

Updated: Apr 18, 2026

Dissection and Culture of Mouse Embryonic Kidney

Dissection and Culture of Mouse Embryonic Kidney

Published on: May 17, 2017

Claudin-7, -16, and -19 during mouse kidney development.

Halim Khairallah¹, Jasmine El Andalousi², Annie Simard²

¹Department of Human Genetics; McGill University ; Montreal, Quebec, Canada.

Tissue Barriers

|January 23, 2015

Summary

Claudin proteins are crucial for kidney development. While Claudin-7, Claudin-16, and Claudin-19 are expressed during kidney development, their absence did not affect nephron formation in mice.

Keywords:

E, embryonic day FHHNC, familial hypomagnesaemia with hypercalciuria and nephrocalcinosis Loop of Henle MET, mesenchymal to epithelial transition P, postnatal nd, nephric duct nephrogenesis renal branching morphogenesis tubule differentiation ub, ureteric bud

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Area of Science:

Molecular Biology
Developmental Biology
Renal Physiology

Background:

Claudins are essential tight junction proteins regulating epithelial barriers and paracellular transport.
Understanding claudin roles in kidney development is crucial for renal physiology research.

Purpose of the Study:

To investigate the expression and function of specific claudins during mouse kidney development.
To determine the impact of Claudin-7, Claudin-16, and Claudin-19 on nephron formation.

Main Methods:

RT-PCR analysis to identify claudin expression in embryonic kidneys.
Whole mount in situ hybridization and immunohistochemistry for Claudin-7 localization.
Analysis of mutant mouse models lacking Claudin-7 or Claudin-16.

Main Results:

Twenty-three claudin family members were expressed in E13.5 mouse kidneys.
Claudin-7 localized to the nephric duct and ureteric bud derivatives.
Claudin-16 and Claudin-19 were found in developing Loop of Henle tubules.
Mice lacking Claudin-7 or Claudin-16 showed no renal malformations or decreased nephron numbers.

Conclusions:

Claudin-7, Claudin-16, and Claudin-19 exhibit distinct expression patterns during kidney development.
Loss of Claudin-7 or Claudin-16 does not impair de novo nephron formation.
These claudins likely regulate paracellular transport in mature nephrons rather than development.