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Mammalian cell cryopreservation by using liquid marbles.

M Concepción Serrano1, Stefania Nardecchia, María C Gutiérrez

  • 1Instituto de Ciencia de Materiales de Madrid, Consejo Superior de Investigaciones Científicas (CSIC) , C/Sor Juana Inés de la Cruz 3, 28049 Madrid, Spain.

ACS Applied Materials & Interfaces
|January 23, 2015
PubMed
Summary

Liquid marbles offer a novel, non-toxic method for cryopreserving mammalian cells. This technique successfully preserves cell viability and function without harmful cryopreservants, paving the way for new biomedical applications.

Keywords:
L929 fibroblastPTFEcryopreservationliquid marblemammalian cell

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Area of Science:

  • Biotechnology
  • Materials Science
  • Cell Biology

Background:

  • Liquid marbles (LMs) are particle-coated droplets with unique non-adhesive properties.
  • LMs have shown promise in various biomedical applications, including as microbioreactors.
  • Conventional cell cryopreservation often involves toxic cryopreservants.

Purpose of the Study:

  • To investigate the use of liquid marbles for mammalian cell cryopreservation.
  • To evaluate LMs as a non-toxic alternative to traditional cryopreservation methods.
  • To determine optimal conditions for cell viability and recovery using LMs.

Main Methods:

  • Preparation of liquid marbles using hydrophobic powder (poly(tetrafluoroethylene)).
  • Encapsulation of murine L929 fibroblasts within liquid marbles.
  • Cryopreservation and subsequent thawing of cell-laden liquid marbles.
  • Assessment of cell viability, adhesion, morphology, proliferation, and cell cycle post-thaw.

Main Results:

  • Successful cryopreservation and recovery of mammalian cells using liquid marbles within a defined range of droplet volumes and cell densities.
  • No significant adverse effects on major cellular parameters (adhesion, morphology, viability, proliferation, cell cycle) were observed.
  • Demonstrated the potential for LMs to maintain cellular integrity during cryopreservation.

Conclusions:

  • Liquid marbles provide a viable, non-toxic platform for mammalian cell cryopreservation.
  • This method offers a promising alternative to conventional cryopreservation techniques.
  • Further development of cell-containing LMs could advance miniaturized cytotoxicity testing and cell-based assays.