Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Epigenetic Regulation01:37

Epigenetic Regulation

4.3K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
4.3K
Epigenetic Regulation01:46

Epigenetic Regulation

34.6K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
34.6K
Epigenetic Regulation01:46

Epigenetic Regulation

26.7K
26.7K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

10.9K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
10.9K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

9.1K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
9.1K
Histone Modification02:32

Histone Modification

18.0K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
18.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The high explosives & affected targets (HEAT) dataset.

Data in brief·2026
Same author

Advanced and metastatic prostate cancer: ESMO Clinical Practice Guideline for diagnosis, treatment and follow-up.

Annals of oncology : official journal of the European Society for Medical Oncology·2026
Same author

Local and locoregional prostate cancer: ESMO Clinical Practice Guideline for diagnosis, treatment and follow-up.

Annals of oncology : official journal of the European Society for Medical Oncology·2025
Same author

Dual-Baseline Search for Active-to-Sterile Neutrino Oscillations in NOvA.

Physical review letters·2025
Same author

Search for CP-Violating Neutrino Nonstandard Interactions with the NOvA Experiment.

Physical review letters·2024
Same author

A phase II study (AARDVARC) of AZD4635 in combination with durvalumab and cabazitaxel in patients with progressive, metastatic, castration-resistant prostate cancer.

ESMO open·2024

Related Experiment Video

Updated: Apr 18, 2026

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

7.1K

NF-κB-dependent and -independent epigenetic modulation using the novel anti-cancer agent DMAPT.

H Nakshatri1, H N Appaiah2, M Anjanappa2

  • 11] Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, USA [2] Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN, USA.

Cell Death & Disease
|January 23, 2015
PubMed
Summary

Dimethylaminoparthenolide (DMAPT) is an epigenetic modulator that targets nuclear factor-kappaB (NF-κB) pathways. This study reveals DMAPT reverses cancer-specific epigenetic abnormalities by altering histone trimethylation levels.

More Related Videos

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

2.2K
Epigenetic Engineering of K562 Cells: Dual-Vector Episomal Strategy for Stable Targeted DNA Methylation using dCas9-DNMT3A and -HDAC1 Fusion Proteins
09:56

Epigenetic Engineering of K562 Cells: Dual-Vector Episomal Strategy for Stable Targeted DNA Methylation using dCas9-DNMT3A and -HDAC1 Fusion Proteins

Published on: October 31, 2025

665

Related Experiment Videos

Last Updated: Apr 18, 2026

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

7.1K
In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

2.2K
Epigenetic Engineering of K562 Cells: Dual-Vector Episomal Strategy for Stable Targeted DNA Methylation using dCas9-DNMT3A and -HDAC1 Fusion Proteins
09:56

Epigenetic Engineering of K562 Cells: Dual-Vector Episomal Strategy for Stable Targeted DNA Methylation using dCas9-DNMT3A and -HDAC1 Fusion Proteins

Published on: October 31, 2025

665

Area of Science:

  • Oncology
  • Epigenetics
  • Molecular Biology

Background:

  • Constitutive nuclear factor-kappaB (NF-κB) activity is prevalent in many cancers, making it a therapeutic target.
  • Dimethylaminoparthenolide (DMAPT), a water-soluble parthenolide analog, inhibits NF-κB and exhibits anti-tumor effects.

Purpose of the Study:

  • To investigate DMAPT's epigenetic modulation mechanisms, both dependent and independent of NF-κB.
  • To explore the link between DMAPT, NF-κB, histone modifications, and gene expression in cancer.

Main Methods:

  • Utilized genetic ablation of NF-κB p65 subunit and overexpression of super-repressor IκBα.
  • Assessed histone H3K36 trimethylation (H3K36me3) and H4K20 trimethylation levels.
  • Quantified expression of histone trimethylases NSD1, SETD2, and KMT5C.

Main Results:

  • DMAPT and NF-κB inhibition elevated H3K36me3 by increasing NSD1 and SETD2 levels, indicating NF-κB represses these genes.
  • NF-κB activity inversely correlated with NSD1 and H3K36me3 levels.
  • DMAPT also induced H4K20 trimethylation via KMT5C, independent of NF-κB.

Conclusions:

  • DMAPT functions as an epigenetic modulator targeting both NF-κB-dependent and -independent pathways.
  • DMAPT reverses cancer-associated epigenetic abnormalities, including altered histone trimethylation.
  • DMAPT shows promise as a therapeutic agent for reversing epigenetic dysregulation in cancer.