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'Human bocavirus in Pakistani children with gastroenteritis'
Muhammad Masroor Alam1, Adnan Khurshid, Shahzad Shaukat
1Department of Virology, National Institute of Health, Islamabad, Pakistan; Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan.
Journal of Medical Virology
|January 23, 2015
Summary
Human Bocavirus (HBoV) in Pakistan is often co-infected with rotavirus in children with acute diarrhea. This study highlights HBoV
Area of Science:
- Virology and Epidemiology
- Gastroenterology
- Pediatrics
Background:
- Human Bocaviruses (HBoV) are detected globally in respiratory and gastrointestinal infections.
- Four genotypes (HBoV1-4) exist; HBoV-1 is linked to respiratory illness, while HBoV-2, -3, and -4 are implicated in gastroenteritis, though their exact role is unclear.
- Epidemiological data on HBoV in Pakistan, particularly concerning acute diarrhea, is limited.
Purpose of the Study:
- To investigate the genetic diversity and prevalence of HBoV in hospitalized Pakistani children suffering from acute diarrhea.
- To understand the association of HBoV genotypes with gastroenteritis in the Pakistani pediatric population.
- To determine the co-infection patterns of HBoV with other enteric pathogens, specifically rotavirus.
Main Methods:
- Collection of 365 stool samples from hospitalized children with gastrointestinal symptoms in Rawalpindi, Pakistan, during 2009.
- Testing samples for HBoV using real-time RT-PCR and for rotavirus using ELISA.
- Phylogenetic analysis of detected HBoV strains.
Main Results:
- HBoV was detected in 13% (47/365) of samples, with HBoV-1 being the most prevalent genotype (94%).
- A high rate of co-infection (98%) was observed between HBoV and rotavirus.
- HBoV detection was more frequent in males and children aged 7.57 ± 5.4 months; all infected children recovered.
Conclusions:
- This is the first epidemiological study of HBoV in acute gastroenteritis in Pakistan.
- HBoV appears to be frequently co-infected with rotavirus in children with acute diarrhea in Pakistan.
- HBoV alone is not significantly associated with gastroenteritis; co-infection with rotavirus is predominant.
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