Related Experiment Video
Updated: Apr 18, 2026

08:42
Isolating Bronchial Epithelial Cells from Resected Lung Tissue for Biobanking and Establishing Well-Differentiated Air-Liquid Interface Cultures
Published on: May 26, 2023
4.3K
Sequential bilateral lung isolation with a single bronchial blocker.
Jay B Brodsky1, Alex Tzabazis, Jennifer Basarb-Tung
1From the Departments of *Anesthesia and †Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, California.
A & a Case Reports
|January 23, 2015
Summary
This study demonstrates a novel method for sequential bilateral lung collapse using a single bronchial blocker (BB), eliminating the need for repositioning during surgery. This simplifies lung separation procedures for thoracic operations.
Area of Science:
- Anesthesiology
- Thoracic Surgery
- Respiratory Physiology
Background:
- Lung separation is crucial for thoracic surgery, enabling selective lung ventilation and collapse.
- Current methods include double-lumen tubes or repositioning single/double bronchial blockers (BBs).
- Repositioning BBs can complicate surgical procedures and increase operative time.
Purpose of the Study:
- To present a technique for sequential bilateral lung collapse using a single bronchial blocker (BB) without repositioning.
- To evaluate the feasibility and efficiency of this simplified approach for lung isolation.
Main Methods:
- Utilized a single bronchial blocker (BB) placed in the main bronchus for sequential lung collapse.
- Developed a method to achieve bilateral lung isolation without requiring intraoperative BB repositioning.
- Applied this technique during thoracic surgical procedures requiring lung separation.
Main Results:
- Successfully achieved sequential bilateral lung collapse with a single bronchial blocker (BB).
- Demonstrated that repositioning of the bronchial blocker (BB) was not necessary during the operation.
- The technique proved effective for lung isolation in the context of thoracic surgery.
Conclusions:
- A single bronchial blocker (BB) can be effectively used for sequential bilateral lung collapse without repositioning.
- This method offers a simplified and potentially more efficient approach to lung separation in thoracic surgery.
- Further investigation may confirm the widespread applicability and benefits of this technique.

