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Updated: Apr 18, 2026
ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection
Published on: October 23, 2009
[Non-modified magnetic beads coupled with multiple real-time PCR for detection and quantification of mycotoxigenic
Yan Jin1, Wei-Wei Zhang, Su-Yuan Wang
1Department of Public Health Laboratory Sciences, West China School of Public Health, Sichuan University, Chengdu 610041, China.E-mail: edrollanca@163.com.
Objective:
To establish a method for detecting 3 common toxigenic molds (Aspergillus, Penicillium, and Fusarium) based on non-modified magnetic beads coupled with multiple real-time PCR (NMB-multiple qPCR).
Methods:
The primers and genus-specific probes were designed based on the rDNA sequences to develop a multiple real-time PCR using non-modified magnetic bead to enrichment of fungal spores. The sensitivity, specificity and repeatability of this assay were evaluated.
Results:
The detection limit of this assay for spiked samples was 10(4) CFU/g, demonstrating a 10-fold greater detection sensitivity of this assay than that of real-time PCR. The NMB-multiple qPCR assay also showed good specificity and reproducibility and yielded comparable results with those by traditional colony counting method for spiked samples (P>0.05).
Conclusion:
NMB-multiple qPCR assay we established allows rapid and sensitive detection of common mycotoxigenic fungi in paprika.
