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Related Concept Videos

Proteomics01:33

Proteomics

10.2K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
10.2K

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Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
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Proteomics. Tissue-based map of the human proteome.

Mathias Uhlén1, Linn Fagerberg2, Björn M Hallström3

  • 1Science for Life Laboratory, KTH-Royal Institute of Technology, SE-171 21 Stockholm, Sweden. Department of Proteomics, KTH-Royal Institute of Technology, SE-106 91 Stockholm, Sweden. Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, DK-2970 Hørsholm, Denmark. mathias.uhlen@scilifelab.se.

Science (New York, N.Y.)
|January 24, 2015
PubMed
Summary

This study maps the human tissue proteome, revealing protein expression across 32 organs. The findings enhance understanding of human biology and disease through a comprehensive, interactive database.

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Production of Tissue Microarrays, Immunohistochemistry Staining and Digitalization Within the Human Protein Atlas
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Production of Tissue Microarrays, Immunohistochemistry Staining and Digitalization Within the Human Protein Atlas

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Area of Science:

  • Human Molecular Biology
  • Proteomics
  • Genomics

Background:

  • Understanding human biology and disease requires detailed knowledge of proteome variation across tissues.
  • Previous studies lacked comprehensive, spatially resolved protein data for the entire human body.

Purpose of the Study:

  • To create a comprehensive map of the human tissue proteome.
  • To spatially localize proteins at the single-cell level across 32 human tissues and organs.
  • To explore specific proteomic subsets like the secretome, membrane proteome, and cancer proteome.

Main Methods:

  • Integrated omics approach combining quantitative transcriptomics and tissue microarray-based immunohistochemistry.
  • Analysis of protein expression across 32 distinct human tissues and organs.
  • Development of an interactive web-based database for data exploration.

Main Results:

  • Detected over 90% of putative protein-coding genes across the analyzed tissues.
  • Provided spatial localization of proteins down to the single-cell level.
  • Explored the human secretome, membrane proteome, druggable proteome, cancer proteome, and metabolic functions.

Conclusions:

  • The human tissue proteome map provides unprecedented insight into human biology and disease.
  • The integrated database facilitates exploration of protein expression patterns across all major human tissues and organs.
  • This resource is valuable for research in various fields, including drug discovery and cancer biology.