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Ag/FeCo/Ag core/shell/shell magnetic nanoparticles with plasmonic imaging capability.

Mari Takahashi1, Priyank Mohan, Akiko Nakade

  • 1School of Materials Science, Japan Advanced Institute of Science and Technology , 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan.

Langmuir : the ACS Journal of Surfaces and Colloids
|January 24, 2015
PubMed
Summary

Novel silver/iron-cobalt/silver nanoparticles (Ag/FeCo/Ag NPs) enable magnetic separation of subcellular components. Their silver core allows easy detection via plasmon scattering, offering an alternative to fluorescent probes.

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Area of Science:

  • Nanotechnology
  • Biotechnology
  • Materials Science

Background:

  • Magnetic nanoparticles (NPs) are utilized for separating biological entities like cells and proteins.
  • Existing methods for subcellular component separation often rely on fluorescent probes, which can have limitations.

Purpose of the Study:

  • To synthesize and characterize Ag/FeCo/Ag core/shell/shell NPs for magnetic separation of subcellular components, such as intracellular vesicles.
  • To evaluate the potential of these NPs as an alternative to traditional fluorescent probes.
  • To investigate the biocompatibility and imaging capabilities of the functionalized NPs.

Main Methods:

  • Synthesis of Ag/FeCo/Ag core/shell/shell NPs.
  • Surface functionalization with ε-poly-L-lysine-based hydrophilic polymers for water-solubility and biocompatibility.
  • Investigation of imaging capabilities using plasmon scattering.
  • Assessment of magnetic response using liposomes as platforms and confocal laser scanning microscopy.
  • Magnetophoresis experiments to determine magnetic force on liposomes.

Main Results:

  • The synthesized Ag/FeCo/Ag NPs demonstrated magnetic separation capabilities for liposomes.
  • The silver core facilitated detection through plasmon scattering, offering semipermanent observation.
  • The NPs exhibited biocompatibility and water-solubility after polymer functionalization.
  • Electron transfer from the silver core to the iron-cobalt shell suppressed oxidation, enhancing NP stability.
  • Magnetophoresis experiments provided quantitative data on the magnetic forces acting on liposomes.

Conclusions:

  • Ag/FeCo/Ag NPs are a promising tool for the magnetic separation and imaging of subcellular components.
  • These NPs offer advantages over fluorescent probes due to their detection mechanism and stability.
  • The developed NPs represent a significant advancement in magnetic separation technology for biological applications.