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Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells

Published on: May 28, 2021

A national facility for biological cryo-electron microscopy.

Helen R Saibil¹, Kay Grünewald², David I Stuart²

¹Crystallography, Institute for Structural and Molecular Biology, Birkbeck College, Malet Street, London WC1E 7HX, England.

Acta Crystallographica. Section D, Biological Crystallography

|January 24, 2015

Summary

A new UK national facility will provide advanced three-dimensional electron microscopy (3D-EM) for structural biology research. This resource will offer state-of-the-art equipment and expert support for detailed molecular and cellular analysis.

Keywords:

three-dimensional electron microscopy

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Area of Science:

Structural Biology
Biophysics
Cell Biology

Background:

Three-dimensional electron microscopy (3D-EM) offers atomic-level insights into cellular machinery, disease mechanisms, and pathogen actions.
High-end 3D-EM research demands significant investment in equipment, infrastructure, and specialized expertise.

Purpose of the Study:

To outline plans for a UK national 3D-EM facility for integrated structural biology.
To enable world-leading research into the fundamental machinery of life.

Main Methods:

Establishment of a national facility with state-of-the-art equipment.
Provision of expert support for both single-particle analysis and cryo-tomography.
Integration with the Diamond Light Source for streamlined access and user-friendly workflows.

Main Results:

The facility will provide access to advanced 3D-EM capabilities.
It will support research at resolutions from atomic detail to the cellular context.
User-friendly workflows with near-real-time feedback will be developed.

Conclusions:

The proposed national facility will significantly advance structural biology research in the UK.
It will democratize access to cutting-edge 3D-EM technology.
The facility aims to foster internationally leading research in the machinery of life.