Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antibiotic Selection00:57

Antibiotic Selection

62.4K
Overview
62.4K
Inhibitors of Bacterial Protein Synthesis01:25

Inhibitors of Bacterial Protein Synthesis

81
Aminoglycosides constitute a highly potent class of bactericidal antibiotics that exert their antimicrobial effects by targeting the bacterial ribosome, specifically disrupting protein synthesis. These polycationic molecules consist of amino-modified sugars linked via glycosidic bonds to an aminocyclitol core such as 2-deoxystreptamine or streptamine. Their strong positive charges facilitate tight binding to the negatively charged phosphate backbone of ribosomal RNA (rRNA), primarily at the 16S...
81
Drug Discovery: Overview01:26

Drug Discovery: Overview

13.6K
Drug discovery is a multifaceted process involving extensive screening, testing, and optimization of lead compounds to identify potential new drugs for therapeutic use. It combines several approaches, including screening large numbers of natural products, chemical modification of known active molecules, identification of new drug targets, and rational design based on biological mechanisms and drug-receptor structure. These approaches are carried out in both academic research laboratories and...
13.6K
Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

2.1K
Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
2.1K
Production of Antibiotics01:27

Production of Antibiotics

198
Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...
198
Combined Effects of Drugs: Synergism01:27

Combined Effects of Drugs: Synergism

8.0K
Synergism is a useful mechanism where combining two or more drugs is more effective than each constituent used alone. Such combinations are also called supra-additive interactions. The drugs collectively enhance the final therapeutic effect by acting on different targets. Another advantage is that the low dose of each constituent drug is sufficient to achieve the desired effect. This helps reduce the duration of therapy and lower the adverse effects of these drugs.
Such synergistic combinations...
8.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Central nervous system antituberculosis drug exposures in a rabbit model of tuberculous meningitis.

Antimicrobial agents and chemotherapy·2026
Same author

Rifamycin Structural Modifications Attenuate PXR Binding and CYP3A4 Induction.

Journal of medicinal chemistry·2026
Same author

Cytochrome P450-Mediated Metabolism of Antimycobacterial <i>N</i>α-Aroyl-<i>N</i>-aryl-phenylalanine Amides.

ACS infectious diseases·2026
Same author

Interstitial and recruited macrophages prevent tuberculosis relapse by limiting immune evasion.

EMBO molecular medicine·2026
Same author

Contribution of cytotoxic CD8 T cells, neutrophils and type 1 interferon signaling to hyperinflammation in HIV-associated TB meningitis.

bioRxiv : the preprint server for biology·2026
Same author

Loss-of-Function Mutations in the Penicillin-Binding Protein PonA1 Confer Agar-Dependent Resistance to Durlobactam in <i>Mycobacterium abscessus</i>.

Antibiotics (Basel, Switzerland)·2026

Related Experiment Video

Updated: Apr 18, 2026

Antibiotic Dereplication Using the Antibiotic Resistance Platform
10:49

Antibiotic Dereplication Using the Antibiotic Resistance Platform

Published on: October 17, 2019

11.9K

Antibacterial drug discovery: doing it right.

Martin Gengenbacher1, Thomas Dick2

  • 1Tuberculosis Research Laboratory, Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, Singapore 117545, Republic of Singapore.

Chemistry & Biology
|January 24, 2015
PubMed
Summary

Researchers developed a new screening method to find drugs against Mycobacterium tuberculosis. This approach identified novel inhibitors targeting biotin biosynthesis, a crucial pathway for the bacteria.

More Related Videos

Author Spotlight: Exploring Cytoskeletal Dynamics to Unveil Novel Antibiotics Through Innovative Cell-Based Assays
05:57

Author Spotlight: Exploring Cytoskeletal Dynamics to Unveil Novel Antibiotics Through Innovative Cell-Based Assays

Published on: April 26, 2024

1.4K
Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection
11:56

Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection

Published on: October 25, 2013

14.8K

Related Experiment Videos

Last Updated: Apr 18, 2026

Antibiotic Dereplication Using the Antibiotic Resistance Platform
10:49

Antibiotic Dereplication Using the Antibiotic Resistance Platform

Published on: October 17, 2019

11.9K
Author Spotlight: Exploring Cytoskeletal Dynamics to Unveil Novel Antibiotics Through Innovative Cell-Based Assays
05:57

Author Spotlight: Exploring Cytoskeletal Dynamics to Unveil Novel Antibiotics Through Innovative Cell-Based Assays

Published on: April 26, 2024

1.4K
Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection
11:56

Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection

Published on: October 25, 2013

14.8K

Area of Science:

  • Microbiology
  • Drug Discovery
  • Biochemistry

Background:

  • Developing novel antibacterial strategies is crucial for combating drug-resistant pathogens.
  • Identifying effective hit-target couples is essential for efficient lead compound discovery and optimization.
  • Mycobacterium tuberculosis poses a significant global health challenge, necessitating new therapeutic approaches.

Purpose of the Study:

  • To introduce a novel screening approach for antibacterial drug discovery.
  • To identify new inhibitors of biotin biosynthesis in Mycobacterium tuberculosis.
  • To validate a target-based whole cell screening method.

Main Methods:

  • A target-based whole cell combination screening approach was employed.
  • The screening focused on identifying inhibitors of biotin biosynthesis.
  • Mycobacterium tuberculosis was used as the model organism.

Main Results:

  • The study successfully identified novel inhibitors of biotin biosynthesis.
  • The developed screening approach proved effective in discovering new antibacterial compounds.
  • The findings highlight potential new leads for tuberculosis treatment.

Conclusions:

  • The novel screening strategy is a promising tool for antibacterial drug discovery.
  • Inhibitors of biotin biosynthesis represent a viable therapeutic avenue against Mycobacterium tuberculosis.
  • This work contributes to the ongoing efforts to find new treatments for tuberculosis.