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Overexpression, purification, crystallization and preliminary X-ray crystallographic analysis of SF173 from Shigella
Ha-Neul Kim1, Jeong-Gi An2, Yoo-Sup Lee1
1Department of Molecular Science andTechnology, Ajou University, Suwon, Kyeonggi 443-749, Republic of Korea.
Abstract:
Shigella flexneri is a Gram-negative, anaerobic bacterium in the genus Shigella that can cause diarrhoea in humans. SF173, a hypothetical protein from S. flexneri 5a strain M90T, has been cloned, overexpressed, purified and crystallized as a part of laboratory-scale structural genomics project. The SF173 protein was crystallized using the sitting-drop vapour-diffusion method in the presence of 0.8 M succinic acid pH 7.0 at 293 K. Preliminary X-ray diffraction analysis revealed that the crystal diffracted to 1.47 Å resolution and belonged to space group I432, with unit-cell parameters a=b=c=110.245 Å.
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