Related Experiment Video
Updated: Apr 18, 2026
A Model of Cardiac Remodeling Through Constriction of the Abdominal Aorta in Rats
Published on: December 2, 2016
Specimen recentrifugation and elevated troponin I levels
Simone Canovi1, Daniele Campioli2, Luigi Marcheselli3
1Department of Laboratory Medicine, Clinical Analysis Laboratory, University Hospital of Modena, and simone.canovi@studio.unibo.it.
Objective:
To compare troponin I levels in plasma before and after specimen recentrifugation.
Methods:
Over a period of 24 consecutive days, we recentrifuged 189 plasma specimens in which the troponin I level was higher than the positivity cut off (60 ng/L) and then remeasured the troponin I.
Results:
Two-tailed Wilcoxon matched-pairs test results identified a statistically significant difference between troponin I concentrations before and after recentrifugation (P <.01). For 94 specimens close to the cut-off value (below the 50th percentile among the overall sample of 189 specimens), Passing-Bablok regression analysis showed a median reduction of 10 ng/L (95% confidence interval, -37.5 ng/L - -10.0 ng/L) in the concentrations of troponin I after recentrifugation.
Conclusion:
Specimen recentrifugation is followed by a reduction in troponin I concentrations in specimens with elevated cardiac troponin I, posing a risk for misclassification of patients. Moreover, this practice gives rise to unnecessary prolongation of turnaround time.
More Related Videos
07:40Impact of High-intensity Interval Exercise and Moderate-Intensity Continuous Exercise on the Cardiac Troponin T Level at an Early Stage of Training
Published on: October 10, 2019
09:23Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum
Published on: December 13, 2017
Related Concept Videos
01:20Blood Studies for Cardiovascular System I: Cardiac Biomarkers
The essential diagnostic tools for detecting myocardial necrosis and monitoring individuals suspected of having acute coronary syndrome (ACS) include:
Troponins
Troponins, particularly cardiac troponins I and T, are the most precise and sensitive markers of myocardial injury. They are detectable within 4-6 hours of myocardial injury and remain...
01:30Acute Coronary Syndrome III: Diagnostic Studies
01:30Acute Coronary Syndrome I: Introduction
01:27Myocarditis II: Clinical Features and Diagnostic Tests
01:19Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...