Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

4.0K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
4.0K
Notch Signaling Pathway03:14

Notch Signaling Pathway

6.9K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
6.9K
Notch Signaling Pathway03:14

Notch Signaling Pathway

6.5K
6.5K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.6K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.6K
Mechanism of Angiogenesis01:10

Mechanism of Angiogenesis

7.8K
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
7.8K
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

8.6K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
8.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Hemodynamic effects of synchronous and asynchronous independent lung ventilation with different levels of positive end-expiratory pressure and tidal volumes on unilateral lung injury in dogs].

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases·2010
Same author

[Study on the immuno-effects and influencing factors of Chinese hamster ovary (CHO) cell hepatitis B vaccine among adults, under different dosages].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2010
Same author

Assemblies of fluorine containing bent-shaped liquid crystal molecules studied by using scanning tunneling microscopy.

Journal of nanoscience and nanotechnology·2010
Same author

Carbon nanotubes induce secondary structure changes of bovine albumin in aqueous phase.

Journal of nanoscience and nanotechnology·2010
Same author

[Immunogenicity and protective efficacy of pertactin recombinants against Bordetella bronchiseptica challenge].

Wei sheng wu xue bao = Acta microbiologica Sinica·2010
Same author

[Analysis of the electrocardiographic findings in 288 patients with acute pulmonary thromboembolism].

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases·2010

Related Experiment Video

Updated: Apr 18, 2026

Author Spotlight: Investigating Angiogenesis and Vessel Permeability Through a Modified Matrix Gel Plug Assay
09:03

Author Spotlight: Investigating Angiogenesis and Vessel Permeability Through a Modified Matrix Gel Plug Assay

Published on: June 30, 2023

1.9K

UXT potentiates angiogenesis by attenuating Notch signaling.

Yi Zhou1, Rui Ge2, Rui Wang2

  • 1State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.

Development (Cambridge, England)
|January 25, 2015
PubMed
Summary

UXT protein is essential for blood vessel formation in zebrafish. It acts as a repressor of Notch signaling, crucial for regulating angiogenesis and endothelial cell behavior.

Keywords:
AngiogenesisHUVECsNotch signalingUXTZebrafish

More Related Videos

Stimulation of Notch Signaling in Mouse Osteoclast Precursors
08:01

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Published on: February 28, 2017

8.5K
Generation and Expansion of Human Cardiomyocytes from Patient Peripheral Blood Mononuclear Cells
05:38

Generation and Expansion of Human Cardiomyocytes from Patient Peripheral Blood Mononuclear Cells

Published on: February 12, 2021

4.9K

Related Experiment Videos

Last Updated: Apr 18, 2026

Author Spotlight: Investigating Angiogenesis and Vessel Permeability Through a Modified Matrix Gel Plug Assay
09:03

Author Spotlight: Investigating Angiogenesis and Vessel Permeability Through a Modified Matrix Gel Plug Assay

Published on: June 30, 2023

1.9K
Stimulation of Notch Signaling in Mouse Osteoclast Precursors
08:01

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Published on: February 28, 2017

8.5K
Generation and Expansion of Human Cardiomyocytes from Patient Peripheral Blood Mononuclear Cells
05:38

Generation and Expansion of Human Cardiomyocytes from Patient Peripheral Blood Mononuclear Cells

Published on: February 12, 2021

4.9K

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Cell Biology

Background:

  • Angiogenesis, the formation of new blood vessels, is a complex process regulated by multiple signaling pathways.
  • The Notch signaling pathway plays a critical role in orchestrating angiogenesis.
  • Understanding the molecular regulators of angiogenesis is vital for therapeutic interventions.

Purpose of the Study:

  • To identify novel regulators of angiogenesis.
  • To elucidate the role of UXT protein in zebrafish development and angiogenesis.
  • To investigate the mechanism by which UXT influences Notch signaling.

Main Methods:

  • Zebrafish embryo studies to assess UXT function in intersegmental vessel (ISV) formation.
  • In vitro and in vivo assays to examine gene expression and protein interactions.
  • Analysis of endothelial cell migration, division, and tip cell behavior.
  • Notch signaling pathway blockade experiments.

Main Results:

  • UXT is essential for ISV formation in zebrafish, with UXT deficiency causing shorter ISVs and impaired endothelial cell functions.
  • UXT protein directly binds to Notch signaling target gene promoters.
  • UXT interacts with the Notch intracellular domain (NICD) and inhibits its association with RBP-Jκ, thereby repressing Notch target gene induction.
  • Blocking Notch signaling rescues the angiogenesis defects caused by UXT knockdown.

Conclusions:

  • UXT functions as a novel repressor of Notch signaling.
  • UXT plays a critical role in the molecular regulation of angiogenesis by modulating Notch signaling.
  • These findings provide new insights into the intricate mechanisms governing blood vessel development.