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Association of an HLA-G 14-bp Insertion/Deletion polymorphism with high HBV replication in chronic hepatitis

A B Laaribi1,2, I Zidi1, N Hannachi2

  • 1Laboratory Microorganisms and Active Biomolecules, Sciences Faculty of Tunis, University of Tunis El Manar, Tunis, Tunisia.

Journal of Viral Hepatitis
|January 27, 2015
PubMed

Insights

The Human Leukocyte Antigen-G (HLA-G) 14-bp Insertion/Deletion polymorphism is linked to higher Hepatitis B virus (HBV) DNA levels in chronic HBV infection. This genetic variation may help predict disease activity and outcomes in patients.

Area of Science:

  • Immunogenetics
  • Hepatology
  • Molecular Biology

Background:

  • The Human Leukocyte Antigen-G (HLA-G) plays a crucial role in immune regulation and is implicated in various infections.
  • A known polymorphism in the 3' untranslated region of HLA-G, the 14-bp Insertion/Deletion (Ins/Del), affects mRNA stability and protein levels.
  • Chronic Hepatitis B virus (HBV) infection is a significant global health concern with complex host-pathogen interactions.

Purpose of the Study:

  • To investigate the association between the HLA-G 14-bp Ins/Del polymorphism and susceptibility to chronic HBV infection.
  • To evaluate the relationship between this polymorphism and viral load, disease progression, and fibrosis in HBV-infected patients.

Main Methods:

  • A case-control study was conducted involving 263 patients with chronic HBV infection and 246 healthy controls.
  • Genomic DNA was extracted, and the HLA-G 14-bp Ins/Del polymorphism was genotyped using Polymerase Chain Reaction (PCR).
  • Statistical analyses were performed to assess associations with HBV infection, viral DNA levels, and fibrosis stages.

Main Results:

  • No significant association was found between the HLA-G 14-bp Ins/Del polymorphism and overall susceptibility to HBV infection.
  • A significant association emerged between the Ins allele and higher HBV DNA levels (P = 0.0024, OR = 1.71).
  • The Ins/Ins genotype conferred a 2.5-fold increased risk of high HBV replication compared to other genotypes, while no link to fibrosis stage was observed.

Conclusions:

  • The HLA-G 14-bp Ins/Del polymorphism is not associated with susceptibility to chronic HBV infection.
  • This polymorphism, particularly the Ins allele and Ins/Ins genotype, is significantly associated with enhanced HBV replication (high viral load).
  • The findings suggest a potential prognostic value of the HLA-G 14-bp Ins/Del polymorphism for evaluating disease outcomes in chronic HBV patients.
Keywords:
HLA-GHLA-G 14-bp Insertion/Deletionassociation studychronic hepatitisfibrosishepatitis B virus

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