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Related Experiment Video

Updated: Apr 18, 2026

Electroporation-Based Genetic Modification of Primary Human Pigment Epithelial Cells Using the Sleeping Beauty Transposon System

Electroporation-Based Genetic Modification of Primary Human Pigment Epithelial Cells Using the Sleeping Beauty Transposon System

Published on: February 4, 2021

Electroporation-mediated gene delivery.

Jennifer L Young¹, David A Dean²

¹Department of Pediatrics, University of Rochester, Rochester, NY, USA.

Advances in Genetics

|January 27, 2015

Summary

This summary is machine-generated.

Electroporation uses electrical fields to deliver macromolecules like DNA into cells and tissues, significantly enhancing gene delivery and expression. This versatile technique shows promise for various applications, including cancer treatment and vaccine development.

Keywords:

Electric field Electropermeabilization Electroporation Endocytosis Intracellular trafficking Physical methods Transfection Vaccines

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Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation

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Last Updated: Apr 18, 2026

Electroporation-Based Genetic Modification of Primary Human Pigment Epithelial Cells Using the Sleeping Beauty Transposon System

Electroporation-Based Genetic Modification of Primary Human Pigment Epithelial Cells Using the Sleeping Beauty Transposon System

Published on: February 4, 2021

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Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer in vitro

Published on: September 12, 2011

Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation

Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation

Published on: June 23, 2011

Area of Science:

Biotechnology
Molecular Biology
Cell Biology

Background:

Electroporation has been a key technique for DNA transfer in various cell types for three decades.
Advances include cell fusion, drug delivery, and gene/drug delivery in living tissues.
The method uses electrical fields to transiently permeabilize cell membranes for macromolecule entry.

Purpose of the Study:

To provide an overview of electroporation theory for DNA delivery in cells and tissues.
To discuss applications of electroporation for in vivo gene delivery in animal models.
To highlight the efficacy and versatility of electroporation in various biological contexts.

Main Methods:

Utilizes electrical fields to create transient pores in cell membranes.
Applies specific field strengths to minimize tissue damage during macromolecule delivery.
Involves optimizing electric field parameters, electrode design, and target cells/tissues.

Main Results:

Achieves 100 to 1000-fold increases in gene delivery and expression.
Enhances the distribution and cellular uptake of DNA.
Demonstrates successful in vivo gene delivery in various tissues (muscle, skin, heart, liver, lung, vasculature).

Conclusions:

Electroporation is a powerful tool for in vivo gene delivery with broad applications in medicine and biotechnology.
Optimization of parameters is crucial for maximizing efficacy in different settings.
The technique is effective for vaccine production, cancer treatment, and transgene expression.