Related Experiment Video
Updated: Apr 18, 2026

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
Published on: March 30, 2019
MiR-30a upregulates BCL2A1, IER3 and cyclin D2 expression by targeting FOXL2
Tairen Wang1, Fei Li1, Shengjian Tang1
1Institute of Plastic Surgery, Weifang Medical College, Weifang, Shandong 261041, P.R. China.
Abstract:
FOXL2 is a transcription factor that is essential for ovarian development. Somatic mutations of FOXL2 are associated with ovarian granulosa cell tumorigenesis. In the present study, the expression of FOXL2 was suppressed by microRNAs using the Ago2 knockdown method in COV434 cells. Online bioinformatics tools were utilized to predict that FOXL2 expression may be repressed by miR-30 family members, and dual luciferase assay and western blotting were performed to demonstrate that FOXL2 is a target gene of miR-30a, which is relatively abundant in COV434 cells. Furthermore, miR-30a overexpression upregulates BCL2A1, IER3 and cyclin D2 expression by inhibiting FOXL2. miR-30a is known to function as a tumor suppressor in breast cancer, small cell lung cancer and colorectal carcinoma; however, the present study revealed an opposing function of miR-30a as an oncogene.
Related Concept Videos
Master Transcription Regulators
MicroRNAs
MAPK Signaling Cascades
Mitogens and the Cell Cycle
PI3K/mTOR/AKT Signaling Pathway
Abnormal Proliferation

