Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Bone Marrow Sampling and Transplants

Bone Marrow Sampling and Transplants

Bone marrow transplant is a potential cure for several diseases, including cancer and specific genetic disorders. Notably, this procedure is applicable for patients suffering from aplastic anemia, certain types of leukemia, severe combined immunodeficiency disease (SCID), Hodgkin's disease, non-Hodgkin's lymphoma, multiple myeloma, thalassemia, sickle-cell disease, and certain cancers.
The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Intensive chemotherapy for high-risk acute lymphoblastic leukemia in first remission: results from the NOPHO ALL2008 study.

Leukemia·2025

Same author

DNA-incorporated thioguanine to detect potential non-adherence to maintenance therapy in acute lymphoblastic leukemia.

Cancer chemotherapy and pharmacology·2025

Same author

Glucocorticoid use in paediatric posterior fossa tumour surgery and the occurrence of postoperative speech impairment.

Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery·2025

Same author

Flow cytometric analysis of cerebrospinal fluid identifies patients with occult central nervous system involvement in relapsed pediatric acute lymphoblastic leukemia.

Haematologica·2025

Same author

Early Increase in Weight Is Seen During Treatment for Paediatric Acute Lymphoblastic Leukaemia-A Retrospective Analysis of Medical Records.

Acta paediatrica (Oslo, Norway : 1992)·2025

Same author

A Comparative Study of Bone Marrow and Central Nervous System Microenvironment Models to Improve In Vitro Studies of Acute Lymphoblastic Leukemia.

Hematological oncology·2025

Search research articles

Related Experiment Video

Updated: Apr 18, 2026

Author Spotlight: Advancing Biomedical Research Through Single Cell Analysis

Author Spotlight: Advancing Biomedical Research Through Single Cell Analysis

Published on: December 22, 2023

Archival bone marrow samples: suitable for multiple biomarker analysis.

Bendik Lund, Laeya A Najmi, Agata Wesolowska-Andersen

Applied Immunohistochemistry & Molecular Morphology : AIMM

|January 27, 2015

Summary

Archival bone marrow samples can be used for genetic studies in childhood leukemia. However, whole genome amplification (WGA) and analysis of longer DNA fragments present challenges, requiring method optimization.

More Related Videos

Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies

Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies

Published on: November 10, 2023

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia

Published on: March 5, 2018

Related Experiment Videos

Last Updated: Apr 18, 2026

Author Spotlight: Advancing Biomedical Research Through Single Cell Analysis

Author Spotlight: Advancing Biomedical Research Through Single Cell Analysis

Published on: December 22, 2023

Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies

Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies

Published on: November 10, 2023

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia

Published on: March 5, 2018

Area of Science:

Genetics
Oncology
Molecular Biology

Background:

Archival samples offer valuable genetic insights for severe diseases like cancer.
Childhood acute lymphoblastic leukemia (ALL) presents unique challenges for genetic research.

Purpose of the Study:

To assess the utility of archival bone marrow smears and biopsies for DNA extraction and genetic marker analysis.
To evaluate the impact of sample age and whole genome amplification (WGA) on DNA quality and genotyping success.

Main Methods:

DNA extraction and purification from archival bone marrow samples (smears and biopsies).
Whole genome amplification (WGA) and analysis of short tandem repeats (STRs).
Genotyping of 33,683 single nucleotide polymorphisms (SNPs) using targeted next-generation sequencing.

Main Results:

DNA concentration measurements varied between spectrophotometry and fluorometry.
STR detection rates decreased with longer fragments, especially after WGA.
Samples stored for 0-3 years yielded better results than those stored for 4-10 years.
Acceptable SNP genotyping call rates were achieved for 7 out of 42 archival samples.

Conclusions:

Archival bone marrow samples are suitable for DNA extraction and multiple marker analysis in childhood ALL research.
WGA efficacy is limited, particularly with longer DNA fragments.
Optimized methods are needed for successful comprehensive SNP analysis from archival samples.