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Updated: Jul 28, 2026

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method
Published on: August 6, 2016
Re-purposing bridging flocculation for on-site, rapid, qualitative DNA detection in resource-poor settings
E J H Wee1, H Y Lau, J R Botella
1Centre for Personalized Nanomedicine, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, QLD 4072, Australia. m.trau@uq.edu.au.
Abstract:
Developing molecular diagnostics in resource-poor settings is challenging. As such, we purpose-built a novel bridging flocculation assay for qualitative evaluation of isothermally amplified DNA by naked eye. The flocculation assay was dependent on pH, DNA polymer amounts and lengths. The method was first applied to the rapid and sensitive detection of important plant pathogens and subsequently extended to other pathogens across the animal kingdom to demonstrate the wide applications of our approach.
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