Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange
Related Experiment Video
Updated: Apr 18, 2026

08:54
Bimolecular Fluorescence Complementation
Published on: April 15, 2011
28.8K
Yidan Ding1, Jiao Li2, Jhon Ralph Enterina1
1Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
Nature Methods
|January 27, 2015
Abstract:
We have developed a versatile new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization-dependent fluorescent proteins. We demonstrate the use of this strategy to construct both intermolecular and intramolecular ratiometric biosensors for qualitative imaging of caspase activity, Ca(2+) concentration dynamics and other second-messenger signaling activities.

