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Updated: Apr 18, 2026

Metabolic Profiling to Determine Bactericidal or Bacteriostatic Effects of New Natural Products using Isothermal Microcalorimetry
Published on: October 29, 2020
Structural basis of a temporin 1b analogue antimicrobial activity against Gram negative bacteria determined by CD and
Gaetano Malgieri1, Concetta Avitabile2, Maddalena Palmieri1
1†Dipartimento di Scienze e Tecnologie Ambientali Biologiche e Farmaceutiche, Seconda Università degli studi di Napoli, Via Vivaldi 43, 81100 Caserta, Italy.
Abstract:
We here report an original approach to elucidate mechanisms of action of antimicrobial peptides and derive crucial structural requirements for the design of novel therapeutic agents. The high resolution structure of TB_KKG6A, an antimicrobial peptide designed to amplify the spectrum of action of Temporin B, bound to E. coli is here determined by means of CD and NMR methodologies. We have also defined, through STD analysis, the residues in closer proximity to the bacterial membrane.
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