Related Experiment Video

Updated: Apr 18, 2026

A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors
10:28

A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors

Published on: August 17, 2019

10.7K

Enzyme assay for pyridoxal 5'-phosphate by apo-D-amino acid aminotransferase

Hiroki Ota1, Masuwo Mushiga1, Tohru Yoshimura2

  • 1Department of Applied Molecular Chemistry, College of Industrial Technology, Nihon University, 1-2-1 Izumichou, Narashino, Chiba 275-8575, Japan.

Journal of Bioscience and Bioengineering
|January 28, 2015
PubMed

Keywords:
ApoenzymeEnzyme assayPyridoxal 5′-phosphateUltraviolet irradiationd-Amino acid aminotransferase

More Related Videos

Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening07:37

Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening

Published on: May 13, 2020

2.4K
Direct Detection of the Acetate-forming Activity of the Enzyme Acetate Kinase05:51

Direct Detection of the Acetate-forming Activity of the Enzyme Acetate Kinase

Published on: December 19, 2011

23.8K

Related Concept Videos

Allosteric Proteins-ATCase01:19

Allosteric Proteins-ATCase

Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to  N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
7.0K