Related Experiment Video
Updated: Apr 18, 2026
Mouse Model of Surgical Uterine Injury and Subsequent Pregnancy Outcomes
Published on: June 27, 2025
[Establishment of mouse endometrial injury model by electrocoagulation]
Xiaoxiao Hu1, Xiaona Lin1, Yinshen Jiang1
1Department of Obstetrics & Gynecology, Sir Run Run Shaw Hospital, Hangzhou 310016, China.
Objective:
To establish the murine model of moderate endometrial injury.
Methods:
Electrocoagulation was applied to induce endometrial injury of ICR mice with 0.5 watts power while contralateral uterine cavity acted as control without electrocoagulation. The endometrial histomorphology was observed in 7 days later by microscopy and fetal number of each lateral uterus assessed at 17.5 days after pregnancy.
Results:
At 7 days post-electrocoagulation, the average endometrial thickness of operating side was significantly thinner than that of control side (1.14 ± 0.08 vs 1.88 ± 0.15 mm, P < 0.05). The density of endometrial glands of operating side was significantly lower than that of control side (20 ± 2 vs 32 ± 3 per 100x field, P < 0.05). After pregnancy, the average number of embryos at operating side decreased by 63.1% compared with control (3 ± 2 vs 8 ± 2, P < 0.01).
Conclusion:
The established model of endometrial electrocoagulation injury shows morphologic changes and decreased fertile ability. It has potential uses for animal studies of endometrial injury treatment.
