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Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Published on: April 16, 2019

Native silica nanoparticles are powerful membrane disruptors.

Hend I Alkhammash¹, Nan Li, Rémy Berthier

¹Electronics and Computer Science & Institute for Life Sciences, University of Southampton, Southampton, SO17 1BJ, UK. mdp@ecs.soton.ac.uk.

Physical Chemistry Chemical Physics : PCCP

|January 28, 2015

Summary

Silica nanoparticles interact with lipid membranes, causing damage. Particle size and surface chemistry significantly influence membrane perturbation, with larger particles and native silica showing stronger interactions.

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Area of Science:

Nanotechnology
Materials Science
Biophysics

Background:

Silica nanoparticles (SiNPs) are explored for drug delivery.
SiNPs can cause cell membrane damage and cytotoxicity.
Understanding SiNP-membrane interactions is crucial for safe applications.

Purpose of the Study:

Investigate silica nanosphere interactions with lipid membranes.
Determine the influence of size, surface chemistry, and biocoating on membrane integrity.
Correlate in vitro findings with in vivo cytotoxicity.

Main Methods:

Liposome leakage assays using phosphatidylcholine lipids.
Varying nanoparticle size (50, 200, 500 nm) and surface chemistry (native, aminated, carboxylated).
Assessing effects of protein and lipid bilayer coatings, and anionic lipid inclusion.

Main Results:

Larger SiNPs (200, 500 nm) induced significant dye leakage, suggesting membrane rearrangement.
Smaller SiNPs (50 nm) showed dose-dependent leakage, reduced by surface modification.
Protein and lipid coatings reduced membrane perturbation; anionic lipids decreased leakage.

Conclusions:

Native silica surface chemistry exhibits strong membrane interaction.
Nanoparticle size and surface properties critically affect lipid bilayer integrity.
Findings inform the design of safer silica nanoparticles for biomedical use.