Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Types of RNA01:23

Types of RNA

74.3K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
74.3K
Types of RNA01:20

Types of RNA

16.8K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
16.8K
Homologous Recombination02:31

Homologous Recombination

66.0K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
66.0K
RNA Structure01:19

RNA Structure

8.6K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
8.6K
RNA Structure01:23

RNA Structure

82.2K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
82.2K
Translational Regulation01:29

Translational Regulation

876
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
876

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cracking the (zip)code of dynein-dependent RNA localization.

Nature structural & molecular biology·2026
Same author

Integrase anchors viral RNA to the HIV-1 capsid interior.

Nature·2026
Same author

Capsid flexibility during Ty1 virus-like particle assembly.

bioRxiv : the preprint server for biology·2025
Same author

KHNYN is a manganese-dependent endoribonuclease required for ZAP-mediated antiviral restriction.

Nucleic acids research·2025
Same author

Probing the molecular determinants of Ty1 retrotransposon restriction specificity in yeast.

PLoS genetics·2025
Same author

The human ribosome modulates multidomain protein biogenesis by delaying cotranslational domain docking.

Nature structural & molecular biology·2025

Related Experiment Video

Updated: Apr 18, 2026

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
10:05

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

Published on: March 5, 2019

7.0K

KH-RNA interactions: back in the groove.

Giuseppe Nicastro1, Ian A Taylor1, Andres Ramos2

  • 1Division of Molecular Structure, MRC National Institute for Medical Research, London, UK.

Current Opinion in Structural Biology
|January 28, 2015
PubMed
Summary

The study revisits rules for RNA recognition by the hnRNP K-homology (KH) domain. New findings reveal expanded sequence recognition and combinatorial functions of multiple KH domains for RNA binding and remodeling.

More Related Videos

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

5.9K
An Assay for Quantifying Protein-RNA Binding in Bacteria
07:02

An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

7.1K

Related Experiment Videos

Last Updated: Apr 18, 2026

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
10:05

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

Published on: March 5, 2019

7.0K
Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

5.9K
An Assay for Quantifying Protein-RNA Binding in Bacteria
07:02

An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

7.1K

Area of Science:

  • Molecular Biology
  • Structural Biology
  • Genetics

Background:

  • The hnRNP K-homology (KH) domain is crucial for gene regulation via single-stranded nucleic acid binding.
  • Existing models for KH domain-RNA recognition require updates based on recent discoveries.

Purpose of the Study:

  • To provide a revised understanding of KH domain-RNA recognition mechanisms.
  • To explore the diversity of structural variations within KH domains.
  • To elucidate the combinatorial function of multiple KH domains in RNA interaction.

Main Methods:

  • Structural analysis of KH domains.
  • Functional assays for RNA binding.
  • Investigation of multi-domain interactions.

Main Results:

  • Demonstrated that KH domains recognize a broader sequence landscape than previously understood.
  • Revealed diverse structural expansions of the KH domain fold.
  • Showcased how multiple KH domains cooperate to bind longer RNA motifs and remodel RNA structure.

Conclusions:

  • Advances in understanding KH domain-RNA interactions are significant.
  • New insights facilitate a detailed molecular comprehension of KH domain target selection.