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Micro-electro-mechanical systems/near-infrared validation of different sampling modes and sample sets coupled with

Zhisheng Wu¹, Xinyuan Shi¹, Guang Wan¹

¹Beijing University of Chinese Medicine, Beijing, China.

|January 28, 2015

Summary

Micro-electro-mechanical systems/near-infrared technology is reliable for analyzing Chinese herbal medicine. Integrating sphere sampling modes and wider concentration ranges (1-4%) yield better results than fiber optic probes and narrower ranges (1-2%).

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A Multimodal Wide-Field Fourier-Transform Raman Microscope

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Area of Science:

Analytical Chemistry
Spectroscopy
Chemometrics

Background:

Near-infrared (NIR) spectroscopy is a valuable tool for analyzing traditional Chinese medicine (TCM).
Optimizing sampling modes and sample sets is crucial for reliable quantitative analysis in TCM.
Micro-electro-mechanical systems (MEMS) integrated with NIR technology offer potential for advanced analytical applications.

Purpose of the Study:

To evaluate the reliability of MEMS/NIR technology for analyzing TCM.
To compare the performance of integrating sphere versus fiber optic probe sampling modes.
To assess the impact of different sample concentration ranges on model accuracy.

Main Methods:

Investigated analytical models for integrating sphere and fiber optic probe sampling modes.
Optimized spectral pretreatments and selected wavelength regions using interval partial least squares (i-PLS) and moving window partial least squares (MWPLS).
Validated the method using an accuracy profile and test set validation.

Main Results:

Integrating sphere mode models outperformed fiber optic probe mode models.
Fiber optic probe modes showed increased susceptibility to interference due to weaker incident light intensity.
Models using wider sample concentration ranges (1-4%) performed better than those with narrower ranges (1-2%).
Variable selection did not significantly impact the full spectral model's performance.

Conclusions:

MEMS/NIR technology is a reliable analytical technique for TCM.
Integrating sphere sampling is preferable to fiber optic probes for MEMS/NIR analysis of TCM.
Wider concentration ranges in sample sets enhance model performance.
This study provides guidance for analytical guidelines concerning sampling modes and sample sets in MEMS/NIR analysis of TCM.