Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

An Optimization Method of Production-Distribution in Multi-Value-Chain.

Sensors (Basel, Switzerland)·2023

Same author

LILRB2-containing small extracellular vesicles from glioblastoma promote tumor progression by promoting the formation and expansion of myeloid-derived suppressor cells.

Cancer immunology, immunotherapy : CII·2023

Same author

Pure endoscopic minimally invasive surgery with a non‑expandable tubular retractor for intradural extramedullary spinal tumors.

Experimental and therapeutic medicine·2023

Same author

Stable Expression of dmiR-283 in the Brain Promises Positive Effects in Endurance Exercise on Sleep-Wake Behavior in Aging <i>Drosophila</i>.

International journal of molecular sciences·2023

Same author

Endoscopic endonasal transsphenoidal approach for craniopharyngioma: A case report.

Experimental and therapeutic medicine·2023

Same author

MorphoSim: an efficient and scalable phase-field framework for accurately simulating multicellular morphologies.

NPJ systems biology and applications·2023

Same journal

Mitochondrial-derived peptide Humanin protects granulosa cells under oxidative conditions.

Reproduction (Cambridge, England)·2026

Same journal

Spatial Transcriptomics in Ovarian Biology Technologies, Computational Challenges, and Biological Insights.

Reproduction (Cambridge, England)·2026

Same journal

Toll-like Receptor 4 Knockout Mice are Protected Against PMOS-like Pathogenesis.

Reproduction (Cambridge, England)·2026

Same journal

Evidence That Heat Shock Protein A5 (HSPA5) Plays a Role During Bovine In Vitro Embryo Production.

Reproduction (Cambridge, England)·2026

Same journal

The use of isoflurane to cause multiple large Ca2+ transients in mouse eggs.

Reproduction (Cambridge, England)·2026

Same journal

Fibroblast Growth Factor 9 Promotes Rat Leydig Cell Development via H3K4me3 Histone Modifications.

Reproduction (Cambridge, England)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 18, 2026

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

Published on: February 6, 2018

Ppp2ca knockout in mice spermatogenesis.

Xiaoyun Pan¹, Xia Chen¹, Xin Tong¹

¹Key Laboratory of National Reproductive MedicineDepartment of Cell Biology and Medical Genetics, Nanjing Medical University, Nanjing, Jiangsu 210029, China.

Reproduction (Cambridge, England)

|January 29, 2015

Summary

This summary is machine-generated.

Protein phosphatase 2A (PP2A) catalytic subunit alpha (Ppp2ca) is crucial for male fertility. Conditional knockout of Ppp2ca in mice primordial germ cells resulted in infertility, highlighting its essential role in male reproductive development.

More Related Videos

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

Published on: December 28, 2021

Mouse Round Spermatid Injection

Mouse Round Spermatid Injection

Published on: January 26, 2024

Related Experiment Videos

Last Updated: Apr 18, 2026

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

Published on: February 6, 2018

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

Published on: December 28, 2021

Mouse Round Spermatid Injection

Mouse Round Spermatid Injection

Published on: January 26, 2024

Area of Science:

Molecular Biology
Genetics
Reproductive Biology

Background:

Protein phosphatase 2A (PP2A) is a serine/threonine phosphatase regulating key cellular processes like meiosis, mitosis, and apoptosis.
Aberrant PP2A activity is linked to various diseases.
PPP2CA and PPP2CB encode the PP2AC catalytic subunit.

Purpose of the Study:

To investigate the role of Ppp2ca in male reproductive development.
To generate a conditional knockout (CKO) mouse model for Ppp2ca.
To study the impact of Ppp2ca deficiency in primordial germ cells.

Main Methods:

Generation of a Ppp2ca conditional knockout (CKO) mouse model in C57BL/6J background.
Spatial and temporal-specific knockout of Ppp2ca exon 2 in primordial germ cells at embryonic day 12.5.
Assessment of male fertility in CKO mice.

Main Results:

The Ppp2ca-null mutation induced infertility in male C57BL/6J mice.
Conditional knockout specifically in primordial germ cells was achieved.
The study established a valuable tool for further research.

Conclusions:

Ppp2ca is essential for male fertility.
Targeted deletion of Ppp2ca in primordial germ cells leads to infertility.
The generated CKO mice are instrumental for studying Ppp2ca functions in development and disease.