Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

8.4K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
8.4K
RNA Interference01:23

RNA Interference

29.0K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
29.0K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

19.1K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
19.1K
Leaky Scanning02:28

Leaky Scanning

5.9K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lymph node-targeted mRNA delivery of fine-tuned peptide-nanocomplexes for SARS-CoV-2 Vaccination.

Biomaterials·2026
Same author

Duration of vaccine-induced immunity following MMR2 vaccination in a measles and rubella-eliminated country.

International journal of epidemiology·2026
Same author

Effects of <i>Allium fistulosum</i> L. (Green Onion) Root and <i>Avena sativa</i> L. (Oat) Mixtures (WCO31) on the Height of Children: A Multi-Center, Randomized, Double-Blind, Placebo-Controlled Clinical Trial.

Nutrients·2026
Same author

Correction: Cross-protective efficacy of NA-based mRNA vaccine candidates against seasonal and avian influenza viruses.

Frontiers in microbiology·2026
Same author

Cross-protective efficacy of NA-based mRNA vaccine candidates against seasonal and avian influenza viruses.

Frontiers in microbiology·2026
Same author

Immunogenicity and Protective Effects of an Ag85B Tuberculosis Subunit Vaccine Formulated with Synthetic TLR4 Agonists in BCG-Boosted Mice.

Vaccines·2026

Related Experiment Video

Updated: Apr 18, 2026

Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies
13:52

Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies

Published on: March 19, 2014

23.9K

Small interfering (Si) RNA mediated baculovirus replication reduction without affecting target gene expression.

Han Saem Lee1, Ho Yeon Lee1, You-Jin Kim1

  • 1Division of Respiratory Viruses, Center for Infectious Diseases, National Institute of Health, Korea CDC, 187 Osongsaemyong2-ro, 363-951 Cheongju-si, Chungbuk, South Korea.

Virus Research
|January 29, 2015
PubMed
Summary

Short interfering RNAs (siRNAs) targeting glycoprotein 64 (GP64) effectively reduce baculovirus contamination in recombinant protein production. This method enhances bioprocessing safety without impacting desired protein expression, offering a promising solution for biodrug development.

Keywords:
BaculovirusGlycoprotein 64Single-stranded DNA binding proteinsiRNA

More Related Videos

Transient Expression of Foreign Genes in Insect Cells sf9 for Protein Functional Assay
11:12

Transient Expression of Foreign Genes in Insect Cells sf9 for Protein Functional Assay

Published on: February 22, 2018

13.4K
Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
12:20

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Published on: December 29, 2015

22.2K

Related Experiment Videos

Last Updated: Apr 18, 2026

Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies
13:52

Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies

Published on: March 19, 2014

23.9K
Transient Expression of Foreign Genes in Insect Cells sf9 for Protein Functional Assay
11:12

Transient Expression of Foreign Genes in Insect Cells sf9 for Protein Functional Assay

Published on: February 22, 2018

13.4K
Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
12:20

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Published on: December 29, 2015

22.2K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Virology

Background:

  • The baculovirus expression vector system (BEVS) is crucial for producing recombinant proteins and virus-like particles (VLPs) for biodrugs and vaccines.
  • Baculovirus replication and budding during bioprocessing can lead to residual viral contaminants in final protein products, despite purification efforts.

Purpose of the Study:

  • To develop a method for reducing baculovirus contamination during recombinant protein production in insect cells.
  • To investigate the efficacy of short interfering RNAs (siRNAs) targeting essential viral genes in inhibiting baculovirus replication.

Main Methods:

  • Design and application of siRNAs targeting glycoprotein 64 (GP64) and single-stranded DNA-binding protein (DBP).
  • Assessment of siRNA efficacy through Western blot analysis and measurement of baculovirus replication levels.
  • Comparison of GP64 siRNA, DBP siRNA, and scrambled siRNA treatments.

Main Results:

  • GP64 siRNAs successfully suppressed GP64 expression without affecting recombinant protein production.
  • Both GP64 and DBP siRNAs significantly reduced baculovirus replication compared to control treatments.
  • DBP siRNA, however, also inhibited the expression of recombinant proteins.

Conclusions:

  • GP64-targeting siRNAs offer a viable strategy to minimize baculovirus contamination in recombinant protein bioprocessing.
  • RNA interference (RNAi) systems, including siRNAs and short hairpin RNAs (shRNAs), show potential for improving the safety of biodrugs and vaccines produced via BEVS.
  • Further research is needed to develop insect cell lines with stable expression of interfering RNAs for continuous bioprocessing applications.