Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rising threats of ceftolozane-tazobactam resistance in P. aeruginosa and A. baumannii: Species-, phenotype-, and geography-specific insights from a systematic review and meta-analysis.

Indian journal of medical microbiology·2026
Same author

Identification, Biology, and Bactericide Control of Peach Bacterial Shot Hole in Hebei Province, China.

Microorganisms·2026
Same author

Active-Metal-Rich Intermetallics as a Platform for Tiered Interstitial Electronic States.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Actin-dependent regulation of RSV F-mediated cell-cell fusion revealed by visualizing its spatiotemporal dynamics.

Nanoscale horizons·2026
Same author

Targeting Lactate-Driven Stromal Autophagy via MCT1 Disrupts the Immunosuppressive Niche and Sensitizes Pancreatic Cancer to PD-1 Blockade.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Perceived coordinated biological motion sequences warp time perception.

Cognition·2026

Related Experiment Video

Updated: Apr 18, 2026

Ground State Depletion Super-resolution Imaging in Mammalian Cells
07:55

Ground State Depletion Super-resolution Imaging in Mammalian Cells

Published on: November 5, 2017

7.7K

Revealing the carbohydrate pattern on a cell surface by super-resolution imaging.

Junling Chen1, Jing Gao, Jiazhen Wu

  • 1State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P.R. China. hdWang@ciac.ac.cn.

Nanoscale
|January 30, 2015
PubMed
Summary

This study used super-resolution microscopy to map N-acetylglucosamine (N-GlcNAc) distribution on cell membranes. N-GlcNAc clusters were found associated with lipid rafts, revealing insights into carbohydrate function.

More Related Videos

Super-resolution Imaging of the Natural Killer Cell Immunological Synapse on a Glass-supported Planar Lipid Bilayer
09:56

Super-resolution Imaging of the Natural Killer Cell Immunological Synapse on a Glass-supported Planar Lipid Bilayer

Published on: February 11, 2015

12.9K
Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy
06:51

Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy

Published on: August 2, 2018

7.7K

Related Experiment Videos

Last Updated: Apr 18, 2026

Ground State Depletion Super-resolution Imaging in Mammalian Cells
07:55

Ground State Depletion Super-resolution Imaging in Mammalian Cells

Published on: November 5, 2017

7.7K
Super-resolution Imaging of the Natural Killer Cell Immunological Synapse on a Glass-supported Planar Lipid Bilayer
09:56

Super-resolution Imaging of the Natural Killer Cell Immunological Synapse on a Glass-supported Planar Lipid Bilayer

Published on: February 11, 2015

12.9K
Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy
06:51

Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy

Published on: August 2, 2018

7.7K

Area of Science:

  • Cell Biology
  • Glycobiology
  • Microscopy

Background:

  • Carbohydrates play crucial roles in cell functions like adhesion and signaling.
  • Understanding carbohydrate distribution is key to their function but remains challenging.
  • Current methods lack the resolution to visualize nanoscale carbohydrate patterns.

Purpose of the Study:

  • To visualize the nanoscale distribution of N-acetylglucosamine (N-GlcNAc) on cell membranes.
  • To investigate the relationship between N-GlcNAc distribution and lipid rafts.
  • To demonstrate the utility of super-resolution microscopy in studying cell surface glycans.

Main Methods:

  • Direct stochastic optical reconstruction microscopy (dSTORM) was employed for high-resolution imaging.
  • Dual-color dSTORM was used to simultaneously visualize N-GlcNAc and lipid rafts.
  • Quantitative analysis of N-GlcNAc cluster size and co-localization was performed.

Main Results:

  • N-acetylglucosamine (N-GlcNAc) was visualized in irregular clusters on Vero cell apical membranes.
  • The average N-GlcNAc cluster size was determined to be approximately 0.37 μm².
  • A significant co-localization of N-GlcNAc clusters with lipid rafts was observed.

Conclusions:

  • Super-resolution imaging provides unprecedented detail on cell surface carbohydrate organization.
  • N-GlcNAc is spatially organized in clusters associated with functional lipid raft domains.
  • This reveals a closer relationship between carbohydrate distribution and cellular functions.