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Optimization of the time efficient calcium phosphate coating on electrospun poly(d,l-lactide)
Nathalie Luickx1, Natasja Van Den Vreken1, Jonas Segaert1
1Department of Basic Medical Sciences, Biomaterials Group, Ghent University, De Pintelaan 185 (Building B, 4th Fl), B, 9000, Ghent, Belgium.
Journal of Biomedical Materials Research. Part A
|January 30, 2015
Summary
Coating poly(d,l-lactide) fibers with calcium phosphate (CP) enhances bone tissue engineering scaffolds. An optimized method rapidly deposits CP, significantly improving cell adhesion and proliferation for better bone regeneration.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Biomineralization
Background:
- Fibrous polyester scaffolds are promising for bone tissue engineering.
- Bioactive coatings, such as calcium phosphate (CP), can enhance scaffold integration and osteoconductivity.
- Developing efficient and rapid coating methods is crucial for clinical translation.
Purpose of the Study:
- To optimize a fast procedure for coating electrospun poly(d,l-lactide) (PDLLA) fibers with a bioactive calcium phosphate (CP) layer.
- To investigate the effect of coating parameters on the type and degree of CP deposition.
- To evaluate the in vitro biological response of MC3T3-E1 cells to CP-coated PDLLA scaffolds.
Main Methods:
- PDLLA fibers were activated via ultrasonication in demineralized water.
- CP precipitation was induced through alternate dipping in Ca(2+) and PO4(3-) solutions.
- Coating conditions (cycles, concentration, immersion time) were varied.
- MC3T3-E1 cell viability, adhesion, and proliferation were assessed on coated and uncoated scaffolds.
Main Results:
- A rapid (approx. 30 minutes) CP coating procedure was established.
- Coating conditions influenced the type of deposited CP, yielding carbonated calcium deficient apatite (CDAp) or a mixture of apatite and dicalcium phosphate dihydrate (DCPD).
- All CP-coated PDLLA scaffolds showed significantly higher cell attachment after 7 days compared to uncoated controls.
- Scaffolds with an adequate CDAp coating exhibited superior cell response and morphology.
Conclusions:
- The developed method provides a fast and effective way to create bioactive CP coatings on PDLLA scaffolds.
- Optimized CDAp coating significantly enhances cellular response, indicating improved osteoconductivity.
- These CP-coated PDLLA scaffolds show great potential for bone tissue engineering applications.
